Detailed information of TA system TA181251

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3017105..3017792
• Replicon: chromosome
• Accession: NZ_CP064405
• Organism: Mycobacterium tuberculosis variant bovis BCG strain BCG SL 222 Sofia

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: IRY56_RS14085
• Protein ID: WP_003414064.1
• Coordinates: 3017397..3017792 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: IRY56_RS14080
• Protein ID: WP_003414061.1
• Coordinates: 3017105..3017371 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IRY56_RS14055	3012744..3013646	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
IRY56_RS14060	3013715..3014467	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
IRY56_RS14065	3014711..3014986	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
IRY56_RS14070	3014983..3016605	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
IRY56_RS14075	3016692..3017108	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
IRY56_RS14080	3017105..3017371	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
IRY56_RS14085	3017397..3017792	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
IRY56_RS14090	3017789..3018058	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
IRY56_RS14095	3018068..3019162	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
IRY56_RS14100	3019159..3019578	-	420	Protein_2781	winged helix-turn-helix domain-containing protein	-
IRY56_RS14105	3019652..3020131	-	480	WP_003414073.1	dihydrofolate reductase	-
IRY56_RS14110	3020202..3021002	-	801	WP_003911953.1	thymidylate synthase	-
IRY56_RS14115	3021158..3021895	+	738	WP_011799288.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T181251 WP_003414064.1 NZ_CP064405:c3017792-3017397 [Mycobacterium tuberculosis variant bovis BCG]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T181251 NZ_CP085815:c2866149-2865841 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCAATACATGGTTTATCGCAATAAAGGAAACAGCAAGGCTTATCCTTATTTACTTGATGTGCAAAGCGATATTATTGA
TGAACTGCATACTCGCATGGTTATCCCATTGTTTCCTGTGAGCAGATTGGTAAATAACCCAGTTAAGCGCCTCACGCCCA
CTCTGAATGTGGAAGGTAATGACTATCTGGTGATGACTCACGAAATGGCGAGTATCAGGCTTTCGCAGATTGGTGATGAG
GTGATGGACGTTCGTTCCCACAGGCAAACCATCAAAAATGCTCTCGATTTTATCTTTGACGGGTTCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT181251 WP_003414061.1 NZ_CP064405:c3017371-3017105 [Mycobacterium tuberculosis variant bovis BCG]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT181251 NZ_CP085815:c2866391-2866152 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCCGGGTATGAGTGTACGACATAAAAAAACAGTCAGTGTCACGCTGGAACCTGCATTGCTTCAGCAAGCCCGTGATGC
AGGTATTAATCTGTCAGCGGTGCTTACCGCCGCGCTGAAAGAGGAAATCAGTGCCACAGGCGTTGAACGCTGGAAAGCAG
AAAATAGAGCGGGTTTGCAGGAGCTCAATCGTATTACTGATGAGCACGGCCTTTTATCGGACGATTACAGGACGTTTTAA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64