Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3016692..3017371 Replicon chromosome
Accession NZ_CP064405
Organism Mycobacterium tuberculosis variant bovis BCG strain BCG SL 222 Sofia

Toxin (Protein)


Gene name vapC Uniprot ID P9WF90
Locus tag IRY56_RS14075 Protein ID WP_003414059.1
Coordinates 3016692..3017108 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag IRY56_RS14080 Protein ID WP_003414061.1
Coordinates 3017105..3017371 (-) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IRY56_RS14055 3012744..3013646 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
IRY56_RS14060 3013715..3014467 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
IRY56_RS14065 3014711..3014986 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
IRY56_RS14070 3014983..3016605 - 1623 WP_003414057.1 type I restriction-modification system subunit M -
IRY56_RS14075 3016692..3017108 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 Toxin
IRY56_RS14080 3017105..3017371 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
IRY56_RS14085 3017397..3017792 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin -
IRY56_RS14090 3017789..3018058 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
IRY56_RS14095 3018068..3019162 - 1095 WP_003414068.1 restriction endonuclease subunit S -
IRY56_RS14100 3019159..3019578 - 420 Protein_2781 winged helix-turn-helix domain-containing protein -
IRY56_RS14105 3019652..3020131 - 480 WP_003414073.1 dihydrofolate reductase -
IRY56_RS14110 3020202..3021002 - 801 WP_003911953.1 thymidylate synthase -
IRY56_RS14115 3021158..3021895 + 738 WP_011799288.1 dienelactone hydrolase family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-123)

Antitoxin

(16-56)


Sequences


Toxin        


Download         Length: 139 a.a.        Molecular weight: 15773.00 Da        Isoelectric Point: 7.1294

>T181250 WP_003414059.1 NZ_CP064405:c3017108-3016692 [Mycobacterium tuberculosis variant bovis BCG]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Download         Length: 417 bp

>T181250 NZ_CP085815:c2822140-2821853 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGCTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCTCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT181250 WP_003414061.1 NZ_CP064405:c3017371-3017105 [Mycobacterium tuberculosis variant bovis BCG]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT181250 NZ_CP085815:c2821866-2821564 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAGCATGGTTAAACATAAACGCGGCAATGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 5SV2


Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References