Detailed information of TA system TA181245

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2811136..2811776
• Replicon: chromosome
• Accession: NZ_CP064405
• Organism: Mycobacterium tuberculosis variant bovis BCG strain BCG SL 222 Sofia

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: IRY56_RS12950
• Protein ID: WP_003412970.1
• Coordinates: 2811136..2811555 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: IRY56_RS12955
• Protein ID: WP_003412975.1
• Coordinates: 2811552..2811776 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IRY56_RS12920	2806721..2807443	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
IRY56_RS12925	2807960..2808187	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
IRY56_RS12930	2808184..2808585	+	402	WP_003412963.1	PIN domain-containing protein	-
IRY56_RS12935	2808620..2809540	-	921	WP_011799273.1	restriction endonuclease	-
IRY56_RS12940	2809881..2810126	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
IRY56_RS12945	2810185..2811135	+	951	WP_003911916.1	Lsr2 family protein	-
IRY56_RS12950	2811136..2811555	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
IRY56_RS12955	2811552..2811776	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
IRY56_RS12960	2811807..2814650	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
IRY56_RS12965	2814722..2815123	-	402	WP_003412981.1	hypothetical protein	-
IRY56_RS12970	2815123..2815593	-	471	WP_003412985.1	transcription antitermination factor NusB	-
IRY56_RS12975	2815596..2816159	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T181245 WP_003412970.1 NZ_CP064405:c2811555-2811136 [Mycobacterium tuberculosis variant bovis BCG]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T181245 NZ_CP085814:c4359306-4358893 [Salmonella enterica subsp. enterica serovar Enteritidis]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT181245 WP_003412975.1 NZ_CP064405:c2811776-2811552 [Mycobacterium tuberculosis variant bovis BCG]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT181245 NZ_CP085814:c4359553-4359287 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGACGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09