Detailed information of TA system TA181239

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2229776..2230402
• Replicon: chromosome
• Accession: NZ_CP064405
• Organism: Mycobacterium tuberculosis variant bovis BCG strain BCG SL 222 Sofia

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: IRY56_RS10275
• Protein ID: WP_003410075.1
• Coordinates: 2230004..2230402 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: IRY56_RS10270
• Protein ID: WP_019283586.1
• Coordinates: 2229776..2230003 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IRY56_RS10260	2227815..2228159	-	345	WP_003410065.1	ferredoxin family protein	-
IRY56_RS10265	2228348..2229517	-	1170	WP_003899126.1	ATP-binding protein	-
IRY56_RS10270	2229776..2230003	+	228	WP_019283586.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
IRY56_RS10275	2230004..2230402	+	399	WP_003410075.1	PIN domain nuclease	Toxin
IRY56_RS10280	2230585..2231016	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
IRY56_RS10285	2231117..2231551	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
IRY56_RS10290	2231988..2232167	-	180	Protein_2033	hypothetical protein	-
IRY56_RS10295	2232228..2232872	+	645	WP_078396697.1	transposase	-
IRY56_RS10300	2232826..2233416	+	591	WP_003899131.1	IS110 family transposase	-
IRY56_RS10305	2233544..2234800	-	1257	WP_003410095.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T181239 WP_003410075.1 NZ_CP064405:2230004-2230402 [Mycobacterium tuberculosis variant bovis BCG]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T181239 NZ_CP085814:c1548465-1548208 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAATGCCTCCGGCGTCAGCGTCCGCCACATCAACAGCAAAACCCACATGACCACCTATTACTCACAAATCCCCAGCCT
GCATCTTAAGGGCGACTGGCTGGAAGAAGCGGGATTTAAGACCGGGCGCGGCGTCACCGTGAAGATTTCACAGGGGTGTA
TTGTGCTGATGGCGGACAGTAACGAGGAGCAGAAGCTGCGCGAGCAGCTTTATCAGGCTAAACAGGTGGTTAAGGGAATT
AAGGACGTGCTGGTTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8347.14 Da
• Isoelectric Point: 4.0995

>AT181239 WP_019283586.1 NZ_CP064405:2229776-2230003 [Mycobacterium tuberculosis variant bovis BCG]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSDDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT181239 NZ_CP085814:1548290-1548622 [Salmonella enterica subsp. enterica serovar Enteritidis]
TCCGCCATCAGCACAATACACCCCTGTGAAATCTTCACGGTGACGCCGCGCCCGGTCTTAAATCCCGCTTCTTCCAGCCA
GTCGCCCTTAAGATGCAGGCTGGGGATTTGTGAGTAATAGGTGGTCATGTGGGTTTTGCTGTTGATGTGGCGGACGCTGA
CGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTATCCGCCGGATGCTGGCTGATTGTGGAGCTCAGGGTG
AGCGAGTATGGGCGCGACATCCTGGCACTGAGCCCCCCTCTCCCCGGCCAGCTCCGGGCGGTGATGAGCAACCGGCTGCC
GGGGCCGTATTTT

Structures

Toxin

• PDB: 4CHG