Detailed information of TA system TA181233

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2179093..2179796
• Replicon: chromosome
• Accession: NZ_CP064405
• Organism: Mycobacterium tuberculosis variant bovis BCG strain BCG SL 222 Sofia

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: IRY56_RS09960
• Protein ID: WP_003409778.1
• Coordinates: 2179093..2179422 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: IRY56_RS09965
• Protein ID: WP_003409780.1
• Coordinates: 2179419..2179796 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IRY56_RS09940	2175476..2176546	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
IRY56_RS09945	2176543..2177058	+	516	WP_003409718.1	flavin reductase family protein	-
IRY56_RS09950	2177055..2178116	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
IRY56_RS09955	2178113..2178883	+	771	WP_003409775.1	SDR family oxidoreductase	-
IRY56_RS09960	2179093..2179422	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IRY56_RS09965	2179419..2179796	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
IRY56_RS09970	2179793..2180383	-	591	WP_003910477.1	SEC-C domain-containing protein	-
IRY56_RS09975	2180438..2181802	+	1365	WP_011799233.1	HNH endonuclease	-
IRY56_RS09980	2181957..2182409	-	453	WP_010950636.1	lipoprotein	-
IRY56_RS09985	2182473..2182874	+	402	WP_003409869.1	hypothetical protein	-
IRY56_RS09990	2182867..2183049	-	183	WP_003409870.1	hypothetical protein	-
IRY56_RS09995	2183163..2183513	-	351	WP_003409871.1	hypothetical protein	-
IRY56_RS10000	2183524..2184426	-	903	WP_003409874.1	hypothetical protein	-
IRY56_RS10005	2184447..2184638	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T181233 WP_003409778.1 NZ_CP064405:c2179422-2179093 [Mycobacterium tuberculosis variant bovis BCG]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T181233 NZ_CP085814:c660399-660088 [Salmonella enterica subsp. enterica serovar Enteritidis]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACACTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGCACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATGATCGGGGTAATAAAGAATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT181233 WP_003409780.1 NZ_CP064405:c2179796-2179419 [Mycobacterium tuberculosis variant bovis BCG]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT181233 NZ_CP085814:c660091-659645 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAAGCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTCAAACTACCAGCAGATGCCTTCATCGAGTAG

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8