Detailed information of TA system TA181225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1387185..1387873
• Replicon: chromosome
• Accession: NZ_CP064405
• Organism: Mycobacterium tuberculosis variant bovis BCG strain BCG SL 222 Sofia

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: IRY56_RS06495
• Protein ID: WP_003406304.1
• Coordinates: 1387442..1387873 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: IRY56_RS06490
• Protein ID: WP_003406302.1
• Coordinates: 1387185..1387445 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IRY56_RS06470	1382762..1383586	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
IRY56_RS06475	1383591..1384772	+	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
IRY56_RS06480	1384849..1385949	-	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
IRY56_RS06485	1386120..1387109	+	990	WP_003406301.1	malate dehydrogenase	-
IRY56_RS06490	1387185..1387445	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
IRY56_RS06495	1387442..1387873	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
IRY56_RS06500	1387896..1389584	-	1689	WP_003910308.1	PE family protein	-
IRY56_RS06505	1389764..1390624	+	861	WP_003406306.1	hypothetical protein	-
IRY56_RS06510	1390705..1391535	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
IRY56_RS06515	1391592..1391885	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
IRY56_RS06520	1391882..1392151	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T181225 WP_003406304.1 NZ_CP064405:1387442-1387873 [Mycobacterium tuberculosis variant bovis BCG]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T181225 NZ_CP085813:c4057079-4056768 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGAGGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT181225 WP_003406302.1 NZ_CP064405:1387185-1387445 [Mycobacterium tuberculosis variant bovis BCG]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT181225 NZ_CP085813:c4056767-4056477 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6