Detailed information of TA system TA181224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1242469..1243037
• Replicon: chromosome
• Accession: NZ_CP064405
• Organism: Mycobacterium tuberculosis variant bovis BCG strain BCG SL 222 Sofia

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: IRY56_RS05850
• Protein ID: WP_003405865.1
• Coordinates: 1242663..1243037 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: IRY56_RS05845
• Protein ID: WP_003405863.1
• Coordinates: 1242469..1242666 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IRY56_RS05825	1238510..1239148	-	639	WP_011799158.1	lipid droplet-associated protein	-
IRY56_RS05830	1239238..1240245	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
IRY56_RS05835	1240262..1241245	-	984	WP_003898731.1	hypothetical protein	-
IRY56_RS05840	1241308..1242381	+	1074	WP_003405860.1	redox-regulated ATPase YchF	-
IRY56_RS05845	1242469..1242666	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
IRY56_RS05850	1242663..1243037	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
IRY56_RS05855	1243240..1243938	+	699	WP_010950494.1	hypothetical protein	-
IRY56_RS05860	1244056..1244241	+	186	WP_003901093.1	hypothetical protein	-
IRY56_RS05865	1244168..1244443	-	276	WP_003405867.1	hypothetical protein	-
IRY56_RS05870	1244686..1245009	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
IRY56_RS05875	1245024..1245734	-	711	WP_003911399.1	hypothetical protein	-
IRY56_RS05880	1245756..1245965	+	210	WP_003911400.1	hypothetical protein	-
IRY56_RS05885	1245917..1246624	-	708	Protein_1159	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T181224 WP_003405865.1 NZ_CP064405:1242663-1243037 [Mycobacterium tuberculosis variant bovis BCG]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T181224 NZ_CP085813:c3747836-3747345 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGATCTCCACCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATAGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGCGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCTCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGGTTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTCGCTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGTGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTATCAGCGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT181224 WP_003405863.1 NZ_CP064405:1242469-1242666 [Mycobacterium tuberculosis variant bovis BCG]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT181224 NZ_CP085813:c3748126-3747833 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATTGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACTGACTTTGTGCTGGAAGCTGCCCGTGCTGCCGCCGAGGAAGCGCTGA
TCGAACAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGAGTTTCTCGTTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07