Detailed information of TA system TA181215

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 754673..755306
• Replicon: chromosome
• Accession: NZ_CP064405
• Organism: Mycobacterium tuberculosis variant bovis BCG strain BCG SL 222 Sofia

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQD6
• Locus tag: IRY56_RS03445
• Protein ID: WP_003403365.1
• Coordinates: 754673..755056 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ56
• Locus tag: IRY56_RS03450
• Protein ID: WP_003403368.1
• Coordinates: 755151..755306 (-)
• Length: 52 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IRY56_RS03420	749965..750501	+	537	WP_003403341.1	50S ribosomal protein L10	-
IRY56_RS03425	750538..750930	+	393	WP_003403353.1	50S ribosomal protein L7/L12	-
IRY56_RS03430	750923..751618	-	696	WP_003403355.1	TetR/AcrR family transcriptional regulator	-
IRY56_RS03435	751689..753194	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
IRY56_RS03440	753275..754285	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
IRY56_RS03445	754673..755056	-	384	WP_003403365.1	ribonuclease VapC6	Toxin
IRY56_RS03450	755151..755306	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
IRY56_RS03455	755382..756098	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
IRY56_RS03460	756374..756682	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
IRY56_RS03465	756669..756914	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	-
IRY56_RS03470	757024..757461	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	-
IRY56_RS03475	757458..757712	-	255	WP_003911263.1	antitoxin VapB7	-
IRY56_RS03480	757826..760189	+	2364	WP_003403397.1	arylsulfatase AtsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-44)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13959.73 Da
• Isoelectric Point: 6.0803

>T181215 WP_003403365.1 NZ_CP064405:c755056-754673 [Mycobacterium tuberculosis variant bovis BCG]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRWIDSAQHRLARAGAVG
ALSVVDLLICDTAAARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Nucleotide

• Length: 384 bp

>T181215 NZ_CP085813:359411-359824 [Salmonella enterica subsp. enterica serovar Enteritidis]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTAA

Antitoxin

Protein

• Length: 52 a.a.
• Molecular weight: 5872.72 Da
• Isoelectric Point: 5.7734

>AT181215 WP_003403368.1 NZ_CP064405:c755306-755151 [Mycobacterium tuberculosis variant bovis BCG]
VSVTQIDLDDEALADVMRIAAVHTKKEAVNLAMRDYVERFRRIEALARSRE

Nucleotide

• Length: 156 bp

>AT181215 NZ_CP085813:359164-359430 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGACGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: G0TQD6

Antitoxin

• AlphaFold DB: A0A7U4BSF8