Detailed information of TA system TA181210

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 698319..698965
• Replicon: chromosome
• Accession: NZ_CP064405
• Organism: Mycobacterium tuberculosis variant bovis BCG strain BCG SL 222 Sofia

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: IRY56_RS03120
• Protein ID: WP_003403137.1
• Coordinates: 698319..698732 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: IRY56_RS03125
• Protein ID: WP_003403139.1
• Coordinates: 698729..698965 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IRY56_RS03100	694402..695952	+	1551	WP_003403119.1	MCE family protein	-
IRY56_RS03105	696004..696396	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
IRY56_RS03110	696393..696650	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
IRY56_RS03115	696833..698068	-	1236	WP_003403128.1	ATP-binding protein	-
IRY56_RS03120	698319..698732	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
IRY56_RS03125	698729..698965	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IRY56_RS03130	699069..699575	-	507	WP_003900973.1	two component system sensor kinase HK1	-
IRY56_RS03135	699689..700219	-	531	WP_003917320.1	two component system sensor kinase HK2	-
IRY56_RS03140	700203..700964	-	762	WP_010950420.1	two-component system response regulator TcrA	-
IRY56_RS03145	701021..701332	+	312	WP_003403164.1	hypothetical protein	-
IRY56_RS03150	701404..702354	+	951	WP_003907436.1	DUF1259 domain-containing protein	-
IRY56_RS03155	702595..703179	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
IRY56_RS03160	703181..703891	+	711	Protein_626	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T181210 WP_003403137.1 NZ_CP064405:c698732-698319 [Mycobacterium tuberculosis variant bovis BCG]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T181210 NZ_CP085812:2247583-2247867 [Salmonella enterica subsp. enterica serovar Schwarzengrund]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATTGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT181210 WP_003403139.1 NZ_CP064405:c698965-698729 [Mycobacterium tuberculosis variant bovis BCG]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT181210 NZ_CP085812:2247351-2247593 [Salmonella enterica subsp. enterica serovar Schwarzengrund]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAGGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAGGATGCACTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89