Detailed information of TA system TA181206

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 365128..365771
• Replicon: chromosome
• Accession: NZ_CP064405
• Organism: Mycobacterium tuberculosis variant bovis BCG strain BCG SL 222 Sofia

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WFB8
• Locus tag: IRY56_RS01600
• Protein ID: WP_003401566.1
• Coordinates: 365346..365771 (+)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: IRY56_RS01595
• Protein ID: WP_003401563.1
• Coordinates: 365128..365349 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IRY56_RS01570	360202..361005	-	804	WP_003401540.1	hypothetical protein	-
IRY56_RS01575	361015..362412	-	1398	WP_003401544.1	sulfatase	-
IRY56_RS01580	362591..364411	+	1821	WP_003401546.1	PE family protein	-
IRY56_RS01585	364554..364781	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
IRY56_RS01590	364778..365080	+	303	WP_003401560.1	toxin-antitoxin system toxin	-
IRY56_RS01595	365128..365349	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
IRY56_RS01600	365346..365771	+	426	WP_003401566.1	PIN domain nuclease	Toxin
IRY56_RS01605	365908..366540	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
IRY56_RS01610	366537..367445	+	909	WP_003900117.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-125)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15713.90 Da
• Isoelectric Point: 5.6002

>T181206 WP_003401566.1 NZ_CP064405:365346-365771 [Mycobacterium tuberculosis variant bovis BCG]
VTDQRWLIDKSALVRLTDSPDMEIWSNRIERGLVHITGVTRLEVGFSAECGEIARREFREPPLSAMPVEYLTPRIEDRAL
EVQTLLADRGHHRGPSIPDLLIAATAELSGLTVLHVDKDFDAIAALTGQKTERLTHRPPSA

Nucleotide

• Length: 426 bp

>T181206 NZ_CP085812:c1365066-1364581 [Salmonella enterica subsp. enterica serovar Schwarzengrund]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGCAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTATCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACTTTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT181206 WP_003401563.1 NZ_CP064405:365128-365349 [Mycobacterium tuberculosis variant bovis BCG]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT181206 NZ_CP085812:c1365341-1365054 [Salmonella enterica subsp. enterica serovar Schwarzengrund]
ATGCTATACAAAGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• PDB: 3H87

Antitoxin

• AlphaFold DB: A0A829CG48