Detailed information of TA system TA181198

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4547047..4548305
• Replicon: chromosome
• Accession: NZ_CP064403
• Organism: Pseudomonas aeruginosa strain WTJH12

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9I4U4
• Locus tag: ITG71_RS21070
• Protein ID: WP_003112528.1
• Coordinates: 4547047..4547802 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: ITG71_RS21075
• Protein ID: WP_003086329.1
• Coordinates: 4547937..4548305 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ITG71_RS21060 (ITG71_21045)	4542220..4544763	+	2544	WP_003112526.1	acyl-homoserine-lactone acylase QuiP	-
ITG71_RS21065 (ITG71_21050)	4544918..4546279	-	1362	WP_079391499.1	DNA recombination protein RmuC	-
ITG71_RS21070 (ITG71_21055)	4547047..4547802	-	756	WP_003112528.1	RES family NAD+ phosphorylase	Toxin
ITG71_RS21075 (ITG71_21060)	4547937..4548305	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
ITG71_RS21080 (ITG71_21065)	4548378..4549664	-	1287	WP_023103369.1	FAD-binding oxidoreductase	-
ITG71_RS21085 (ITG71_21070)	4549720..4551309	-	1590	WP_222821302.1	aldehyde dehydrogenase family protein	-
ITG71_RS21090 (ITG71_21075)	4551388..4551864	+	477	WP_003140638.1	hypothetical protein	-
ITG71_RS21095 (ITG71_21080)	4551867..4552142	-	276	WP_169057979.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28278.42 Da
• Isoelectric Point: 7.7294

>T181198 WP_003112528.1 NZ_CP064403:c4547802-4547047 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T181198 NZ_CP085811:622889-623173 [Salmonella enterica subsp. enterica serovar Schwarzengrund]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATTGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT181198 WP_003086329.1 NZ_CP064403:c4548305-4547937 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT181198 NZ_CP085811:622657-622899 [Salmonella enterica subsp. enterica serovar Schwarzengrund]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAGGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAGGATGCACTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: Q9I4U4

Antitoxin

• AlphaFold DB: V6AJU7