Detailed information of TA system TA181197

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3564417..3565638
• Replicon: chromosome
• Accession: NZ_CP064403
• Organism: Pseudomonas aeruginosa strain WTJH12

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ITG71_RS16395
• Protein ID: WP_003114949.1
• Coordinates: 3565060..3565638 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: ITG71_RS16390
• Protein ID: WP_003088112.1
• Coordinates: 3564417..3564971 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ITG71_RS16365 (ITG71_16360)	3559985..3560536	+	552	WP_003132721.1	XRE family transcriptional regulator	-
ITG71_RS16370 (ITG71_16365)	3560533..3560931	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
ITG71_RS16375 (ITG71_16370)	3560944..3561267	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
ITG71_RS16380 (ITG71_16375)	3561598..3562059	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
ITG71_RS16385 (ITG71_16380)	3562064..3564259	+	2196	WP_004349295.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
ITG71_RS16390 (ITG71_16385)	3564417..3564971	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
ITG71_RS16395 (ITG71_16390)	3565060..3565638	+	579	WP_003114949.1	HD domain-containing protein	Toxin
ITG71_RS16400 (ITG71_16395)	3565746..3566933	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
ITG71_RS16405 (ITG71_16400)	3566923..3569094	-	2172	WP_016851733.1	type I secretion system permease/ATPase	-
ITG71_RS16410 (ITG71_16405)	3569084..3570361	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21897.75 Da
• Isoelectric Point: 5.3655

>T181197 WP_003114949.1 NZ_CP064403:3565060-3565638 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAVRRDA

Nucleotide

• Length: 579 bp

>T181197 NZ_CP085811:508049-508540 [Salmonella enterica subsp. enterica serovar Schwarzengrund]
ATGATCTCCGCCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATGGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGTGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAGCGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCTCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGATTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTTGGTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTACCAGCGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT181197 WP_003088112.1 NZ_CP064403:3564417-3564971 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT181197 NZ_CP085811:507759-508052 [Salmonella enterica subsp. enterica serovar Schwarzengrund]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATCGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAAGCCGCCCGCGCTGCCGCAGAGGAAGCGCTGA
TCGAACAACGCATCATTATGGCCGATCCACAGGCTTATCAGGAATTTCTGGCTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA