Detailed information of TA system TA181192

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): slvAT/RES(toxin)
• Location: 150488..151404
• Replicon: plasmid pNDTH10366-KPC
• Accession: NZ_CP064402
• Organism: Pseudomonas aeruginosa strain NDTH10366

Toxin (Protein)

• Gene name: slvT
• Uniprot ID: A0A2L1KI32
• Locus tag: ITG67_RS33305
• Protein ID: WP_019437783.1
• Coordinates: 150488..151012 (-)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: slvA
• Uniprot ID: A0A2L1KH13
• Locus tag: ITG67_RS33310
• Protein ID: WP_019437782.1
• Coordinates: 151009..151404 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ITG67_RS33285 (ITG67_33300)	146340..146954	+	615	Protein_144	glycerate kinase	-
ITG67_RS33290 (ITG67_33305)	147374..147565	-	192	WP_019437785.1	hypothetical protein	-
ITG67_RS33295 (ITG67_33310)	147940..148995	+	1056	WP_139139567.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
ITG67_RS33300 (ITG67_33315)	149192..150442	+	1251	WP_019437784.1	TerD family protein	-
ITG67_RS33305 (ITG67_33320)	150488..151012	-	525	WP_019437783.1	RES family NAD+ phosphorylase	Toxin
ITG67_RS33310 (ITG67_33325)	151009..151404	-	396	WP_019437782.1	hypothetical protein	Antitoxin
ITG67_RS33315 (ITG67_33330)	151988..153871	-	1884	WP_223819158.1	helicase	-
ITG67_RS33320 (ITG67_33335)	154071..154325	+	255	WP_021263738.1	hypothetical protein	-
ITG67_RS33325 (ITG67_33340)	155219..155743	+	525	WP_010794447.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaKPC-2 / sul1 / qacE / aadA1 / cmlA1 / ant(2'')-Ia / aac(6')-Il / blaNDM-14 / dfrA27 / ARR-3 / blaOXA-246 / aac(6')-IIa	-	1..392244	392244

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (10-167)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 19052.57 Da
• Isoelectric Point: 4.5535

>T181192 WP_019437783.1 NZ_CP064402:c151012-150488 [Pseudomonas aeruginosa]
MKIIGVQDLPCYRMHTPKWSVAPTSGAGAATQGGRVNRKGLEALYLSLESATAIAEFQQASAFLPPGLLISYSLSLDRVI
DFTGGYNDTWAPIWQDFFCDWRKIRFNDGVEPPSWIIGDLAIEADCAGILFESVANPGGRNLVLFTDQLPVHGNIVVNDP
RGDLPTDQSSWTRP

Nucleotide

• Length: 525 bp

>T181192 NZ_CP085810:4202680-4203171 [Salmonella enterica subsp. enterica serovar Schwarzengrund]
ATGATCTCCGCCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATGGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGTGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAGCGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCTCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGATTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTTGGTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTACCAGCGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14753.87 Da
• Isoelectric Point: 7.2048

>AT181192 WP_019437782.1 NZ_CP064402:c151404-151009 [Pseudomonas aeruginosa]
MASKAVLELRPTNFAGLMDALKEVNRVMFSPERFLKVFSMDQQTLALRAHVHRNTVRNAPESEKVQAYIRDSVKVLRAVT
DMGTDVTNAIFWFKNEPLSTFGYKTAEEVVSEGKTEQLIAFLQSWEAGAQG

Nucleotide

• Length: 396 bp

>AT181192 NZ_CP085810:4202390-4202683 [Salmonella enterica subsp. enterica serovar Schwarzengrund]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATCGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAAGCCGCCCGCGCTGCCGCAGAGGAAGCGCTGA
TCGAACAACGCATCATTATGGCCGATCCACAGGCTTATCAGGAATTTCTGGCTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Structures

Toxin

• AlphaFold DB: A0A2L1KI32

Antitoxin

• AlphaFold DB: A0A2L1KH13