Detailed information of TA system TA181190

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5703110..5703696
• Replicon: chromosome
• Accession: NZ_CP064401
• Organism: Pseudomonas aeruginosa strain NDTH10366

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: ITG67_RS26710
• Protein ID: WP_003120987.1
• Coordinates: 5703397..5703696 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: ITG67_RS26705
• Protein ID: WP_003448662.1
• Coordinates: 5703110..5703400 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ITG67_RS26690 (ITG67_26705)	5698692..5700581	+	1890	WP_196994395.1	hypothetical protein	-
ITG67_RS26695 (ITG67_26710)	5700578..5702554	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
ITG67_RS35095	5702564..5702698	+	135	WP_255253641.1	hypothetical protein	-
ITG67_RS26700 (ITG67_26715)	5702695..5703039	+	345	WP_016851612.1	hypothetical protein	-
ITG67_RS26705 (ITG67_26720)	5703110..5703400	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
ITG67_RS26710 (ITG67_26725)	5703397..5703696	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ITG67_RS26715 (ITG67_26730)	5703898..5705022	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
ITG67_RS26720 (ITG67_26735)	5705022..5706731	+	1710	WP_003099757.1	PilN family type IVB pilus formation outer membrane protein	-
ITG67_RS26725 (ITG67_26740)	5706735..5708060	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
ITG67_RS26730 (ITG67_26745)	5708050..5708583	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5675079..5764076	88997

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T181190 WP_003120987.1 NZ_CP064401:c5703696-5703397 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T181190 NZ_CP085810:c3837425-3837114 [Salmonella enterica subsp. enterica serovar Schwarzengrund]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCCCTCACTGAGTTGCT
GAATGTTCTTGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATTATCGGGGTAATAAAGAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT181190 WP_003448662.1 NZ_CP064401:c5703400-5703110 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT181190 NZ_CP085810:c3837117-3836671 [Salmonella enterica subsp. enterica serovar Schwarzengrund]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAAGCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTTAAACTACCAGCGGATGCCTTCATCGAGTAG

Structures

Toxin

• AlphaFold DB: G8CP73