Detailed information of TA system TA181189

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4725869..4726509
• Replicon: chromosome
• Accession: NZ_CP064401
• Organism: Pseudomonas aeruginosa strain NDTH10366

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ITG67_RS22130
• Protein ID: WP_003134109.1
• Coordinates: 4725869..4726279 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: ITG67_RS22135
• Protein ID: WP_031634724.1
• Coordinates: 4726279..4726509 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ITG67_RS22085 (ITG67_22085)	4720905..4722230	+	1326	WP_191519258.1	STY4528 family pathogenicity island replication protein	-
ITG67_RS35035	4722227..4722739	+	513	WP_223697281.1	hypothetical protein	-
ITG67_RS22100 (ITG67_22100)	4722896..4723624	+	729	WP_009315862.1	TIGR03761 family integrating conjugative element protein	-
ITG67_RS22105 (ITG67_22105)	4723630..4724160	+	531	WP_121372660.1	DUF3158 family protein	-
ITG67_RS22110 (ITG67_22110)	4724174..4724662	+	489	WP_023104904.1	single-stranded DNA-binding protein	-
ITG67_RS22115 (ITG67_22115)	4725161..4725358	+	198	WP_191519259.1	CrpP family ICE-associated protein	-
ITG67_RS22120 (ITG67_22120)	4725424..4725642	-	219	WP_023083218.1	hypothetical protein	-
ITG67_RS22125 (ITG67_22125)	4725692..4725853	-	162	WP_191519260.1	hypothetical protein	-
ITG67_RS22130 (ITG67_22130)	4725869..4726279	-	411	WP_003134109.1	tRNA(fMet)-specific endonuclease VapC	Toxin
ITG67_RS22135 (ITG67_22135)	4726279..4726509	-	231	WP_031634724.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
ITG67_RS22140 (ITG67_22140)	4726827..4728752	+	1926	WP_191519261.1	type I DNA topoisomerase	-
ITG67_RS22145 (ITG67_22145)	4728766..4729512	+	747	WP_223723588.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	katA	4707406..4837110	129704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-33)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15452.78 Da
• Isoelectric Point: 7.3233

>T181189 WP_003134109.1 NZ_CP064401:c4726279-4725869 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVTPIV

Nucleotide

• Length: 411 bp

>T181189 NZ_CP085810:c3434992-3434507 [Salmonella enterica subsp. enterica serovar Schwarzengrund]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGCAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTATCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACTTTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8420.29 Da
• Isoelectric Point: 4.2821

>AT181189 WP_031634724.1 NZ_CP064401:c4726509-4726279 [Pseudomonas aeruginosa]
VEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVAVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT181189 NZ_CP085810:c3435267-3434980 [Salmonella enterica subsp. enterica serovar Schwarzengrund]
ATGCTATACAAAGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA