Detailed information of TA system TA181180

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4530788..4531428
• Replicon: chromosome
• Accession: NZ_CP064399
• Organism: Pseudomonas aeruginosa strain QZPH41

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ITG68_RS21065
• Protein ID: WP_033993319.1
• Coordinates: 4530788..4531198 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6N0KGL5
• Locus tag: ITG68_RS21070
• Protein ID: WP_023132185.1
• Coordinates: 4531198..4531428 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ITG68_RS30980	4526050..4526733	+	684	WP_226789514.1	hypothetical protein	-
ITG68_RS21030 (ITG68_21020)	4526890..4527618	+	729	WP_023102567.1	TIGR03761 family integrating conjugative element protein	-
ITG68_RS21035 (ITG68_21025)	4527624..4528172	+	549	WP_033993324.1	DUF3158 family protein	-
ITG68_RS21040 (ITG68_21030)	4528220..4529062	+	843	WP_033993323.1	Rha family transcriptional regulator	-
ITG68_RS21045 (ITG68_21035)	4529092..4529580	+	489	WP_033993322.1	single-stranded DNA-binding protein	-
ITG68_RS21050 (ITG68_21040)	4530080..4530277	+	198	WP_071536305.1	CrpP family ICE-associated protein	-
ITG68_RS21055 (ITG68_21045)	4530343..4530561	-	219	WP_033993321.1	hypothetical protein	-
ITG68_RS21060 (ITG68_21050)	4530575..4530772	-	198	WP_003123325.1	hypothetical protein	-
ITG68_RS21065 (ITG68_21055)	4530788..4531198	-	411	WP_033993319.1	tRNA(fMet)-specific endonuclease VapC	Toxin
ITG68_RS21070 (ITG68_21060)	4531198..4531428	-	231	WP_023132185.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
ITG68_RS21075 (ITG68_21065)	4531700..4533619	+	1920	WP_033993318.1	type I DNA topoisomerase	-
ITG68_RS21080 (ITG68_21070)	4533926..4534135	+	210	WP_003105733.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	katA / katA	4512110..4648223	136113

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-33)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15422.76 Da
• Isoelectric Point: 7.3233

>T181180 WP_033993319.1 NZ_CP064399:c4531198-4530788 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVAPIV

Nucleotide

• Length: 411 bp

>T181180 NZ_CP064399:c4531198-4530788 [Pseudomonas aeruginosa]
ATGCTCAAGTTCATGCTCGACACCAACATCTGCATCTTCACCATCAAAAACCGCCCGCAAGAGGTGCGCGAGGCGTTCCT
GCGCCACCACGATCAGATGTGCATCAGCACCGTGACGCTGATGGAGCTGATCTACGGCGCAGAGAAGTCCTCGAACCCCT
CGCGCAATCTTGCGGACGTGGAAGGCTTCGCTGCTCGTCTGGAGGTGCTCAAGTACGACCAGGAAGCTGCCGCCCACACT
GGCCAGCTCCGCGCTGAACTGGCCCGCCTCGGAAAACAAATCGGCCCCTATGACCAGATGATCGCCGGGCACGCCCGCTC
TCAGGGCCTGATCGTTGTCACCAATAACCGCCGCGAGTTCGACCGCGTACCCGGCTTGAGAGTTGAGGATTGGGTCGCCC
CAATCGTGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8468.35 Da
• Isoelectric Point: 4.2821

>AT181180 WP_023132185.1 NZ_CP064399:c4531428-4531198 [Pseudomonas aeruginosa]
MEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVAVGRARIITPAGESWDSWFDGEGVTSDFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT181180 NZ_CP064399:c4531428-4531198 [Pseudomonas aeruginosa]
ATGGAACAAGGTTCTGTTTTTCAGAGCAATCGTAGCCAGGCCGTAAGACTGCCCAAGGCAGTCGCGCTTCCCGATGACGT
TAAGCGTGTGGATGTGGTCGCTGTGGGGCGCGCCAGAATCATCACGCCTGCCGGCGAGTCTTGGGATAGCTGGTTCGATG
GCGAGGGCGTTACTTCCGACTTTATGGCAGAGCGTGAACAGCCTGCCGATCAGGAGCGCGAGACTTTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A6N0KGL5