Detailed information of TA system TA181174

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5653315..5653901
• Replicon: chromosome
• Accession: NZ_CP064397
• Organism: Pseudomonas aeruginosa strain SRRSH1002

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: ITG69_RS26250
• Protein ID: WP_003120987.1
• Coordinates: 5653602..5653901 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: ITG69_RS26245
• Protein ID: WP_003448662.1
• Coordinates: 5653315..5653605 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ITG69_RS26230 (ITG69_26230)	5648897..5650786	+	1890	WP_196994395.1	hypothetical protein	-
ITG69_RS26235 (ITG69_26235)	5650783..5652759	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
ITG69_RS26240 (ITG69_26240)	5652900..5653244	+	345	WP_016851612.1	hypothetical protein	-
ITG69_RS26245 (ITG69_26245)	5653315..5653605	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
ITG69_RS26250 (ITG69_26250)	5653602..5653901	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ITG69_RS26255 (ITG69_26255)	5654103..5655227	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
ITG69_RS26260 (ITG69_26260)	5655227..5656936	+	1710	WP_003099757.1	PilN family type IVB pilus formation outer membrane protein	-
ITG69_RS26265 (ITG69_26265)	5656940..5658265	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
ITG69_RS26270 (ITG69_26270)	5658255..5658788	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5625286..5712498	87212

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T181174 WP_003120987.1 NZ_CP064397:c5653901-5653602 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T181174 NZ_CP085809:45758-46132 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGTCAAAGGGTCTGCGCTTTTTGATACCAATATCCTCATCGACCTGTTTAGCGGTCGAATTGAAGCGAAACATGCGCT
GGAAGCGTATCCGCCACAAAATGCGATTAGCCTTATCACATGGATGGAAGTGATGGTGGGTGCTAAGAAATATCATCAGG
AAAACCGCACGCGCATTGCATTAAGTGCGTTCAATATTATTGGCGTAACACAGGAAATCGCGGAACGAAGCGTCATTGTC
AGGCAGGAGTATGGCATGAAACTCCCGGATGCGATCATTCTGGCCACTGCACAAGTCCATCGTTGTGAGCTTGTGACGCG
CAATACAAAAGACTTTGCCGATATTCCGGGAGTGATCACGCCGTATCACTTGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT181174 WP_003448662.1 NZ_CP064397:c5653605-5653315 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT181174 NZ_CP085809:45516-45758 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGACGATGTGATTAAACGGCTTGACGATCTCAAGGT
GCAACGCAACCTACCTCGTGCAGAACTCTTGCGGGAGGCCGTTGAGCAGTATCTGGAAAGACAGGATCGGGCTGAGACCA
CAATTTCGAAAGCATTGGGGCTCTGGCAAGGCTGTGAGGAAGATGGCGTTGAATACCAACGTAAACTTCGTGAGGAATGG
TAA

Structures

Toxin

• AlphaFold DB: G8CP73