Detailed information of TA system TA181173

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4796626..4797266
• Replicon: chromosome
• Accession: NZ_CP064397
• Organism: Pseudomonas aeruginosa strain SRRSH1002

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ITG69_RS22255
• Protein ID: WP_003134109.1
• Coordinates: 4796626..4797036 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: ITG69_RS22260
• Protein ID: WP_031634724.1
• Coordinates: 4797036..4797266 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ITG69_RS22210 (ITG69_22210)	4791662..4792987	+	1326	WP_191519258.1	STY4528 family pathogenicity island replication protein	-
ITG69_RS32540	4792984..4793496	+	513	WP_223697281.1	hypothetical protein	-
ITG69_RS22225 (ITG69_22225)	4793653..4794381	+	729	WP_009315862.1	TIGR03761 family integrating conjugative element protein	-
ITG69_RS22230 (ITG69_22230)	4794387..4794917	+	531	WP_121372660.1	DUF3158 family protein	-
ITG69_RS22235 (ITG69_22235)	4794931..4795419	+	489	WP_023104904.1	single-stranded DNA-binding protein	-
ITG69_RS22240 (ITG69_22240)	4795918..4796115	+	198	WP_191519259.1	CrpP family ICE-associated protein	-
ITG69_RS22245 (ITG69_22245)	4796181..4796399	-	219	WP_023083218.1	hypothetical protein	-
ITG69_RS22250 (ITG69_22250)	4796449..4796610	-	162	WP_191519260.1	hypothetical protein	-
ITG69_RS22255 (ITG69_22255)	4796626..4797036	-	411	WP_003134109.1	tRNA(fMet)-specific endonuclease VapC	Toxin
ITG69_RS22260 (ITG69_22260)	4797036..4797266	-	231	WP_031634724.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
ITG69_RS22265 (ITG69_22265)	4797584..4799509	+	1926	WP_191519261.1	type I DNA topoisomerase	-
ITG69_RS22270 (ITG69_22270)	4799523..4800269	+	747	WP_223723588.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	katA	4778163..4905011	126848

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-33)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15452.78 Da
• Isoelectric Point: 7.3233

>T181173 WP_003134109.1 NZ_CP064397:c4797036-4796626 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVTPIV

Nucleotide

• Length: 411 bp

>T181173 NZ_CP085809:c44296-43985 [Salmonella enterica subsp. enterica serovar Typhimurium]
GTGCATGTTATCAGCCGAAAACTGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCGCTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATTATCGGGGTAATGAAGAATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8420.29 Da
• Isoelectric Point: 4.2821

>AT181173 WP_031634724.1 NZ_CP064397:c4797266-4797036 [Pseudomonas aeruginosa]
VEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVAVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT181173 NZ_CP085809:c43988-43542 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAAGCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTTAAACTACCAGCGGATGCCTTCATCGAGTAG