Detailed information of TA system TA181166

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5651766..5652352
• Replicon: chromosome
• Accession: NZ_CP064395
• Organism: Pseudomonas aeruginosa strain SRRSH1408

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: ITG72_RS26235
• Protein ID: WP_003120987.1
• Coordinates: 5652053..5652352 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: ITG72_RS26230
• Protein ID: WP_003448662.1
• Coordinates: 5651766..5652056 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ITG72_RS26215 (ITG72_26215)	5647348..5649237	+	1890	WP_196994395.1	hypothetical protein	-
ITG72_RS26220 (ITG72_26220)	5649234..5651210	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
ITG72_RS26225 (ITG72_26225)	5651351..5651695	+	345	WP_016851612.1	hypothetical protein	-
ITG72_RS26230 (ITG72_26230)	5651766..5652056	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
ITG72_RS26235 (ITG72_26235)	5652053..5652352	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ITG72_RS26240 (ITG72_26240)	5652554..5653678	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
ITG72_RS26245 (ITG72_26245)	5653678..5655387	+	1710	WP_003099757.1	PilN family type IVB pilus formation outer membrane protein	-
ITG72_RS26250 (ITG72_26250)	5655391..5656716	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
ITG72_RS26255 (ITG72_26255)	5656706..5657239	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5623737..5710949	87212

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T181166 WP_003120987.1 NZ_CP064395:c5652352-5652053 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T181166 NZ_CP085808:c3462325-3462038 [Salmonella enterica subsp. enterica serovar Typhi]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGCTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCTCGTAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT181166 WP_003448662.1 NZ_CP064395:c5652056-5651766 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT181166 NZ_CP085808:c3462051-3461749 [Salmonella enterica subsp. enterica serovar Typhi]
ATGAGCATGGTTAAACATAAACGCGGCAACGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGACAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGACGTTATGGAATGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTGAATGCCATTCTGCGCGAAGCGATGCTGCGCGAGCAAAATAAGAAATAG

Structures

Toxin

• AlphaFold DB: G8CP73