Detailed information of TA system TA181158

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5547356..5547942
• Replicon: chromosome
• Accession: NZ_CP064393
• Organism: Pseudomonas aeruginosa strain SRRSH1101

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: ITG66_RS25780
• Protein ID: WP_003120987.1
• Coordinates: 5547643..5547942 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: ITG66_RS25775
• Protein ID: WP_003448662.1
• Coordinates: 5547356..5547646 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ITG66_RS25760 (ITG66_25765)	5542938..5544827	+	1890	WP_196994395.1	hypothetical protein	-
ITG66_RS25765 (ITG66_25770)	5544824..5546800	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
ITG66_RS25770 (ITG66_25775)	5546941..5547285	+	345	WP_016851612.1	hypothetical protein	-
ITG66_RS25775 (ITG66_25780)	5547356..5547646	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
ITG66_RS25780 (ITG66_25785)	5547643..5547942	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ITG66_RS25785 (ITG66_25790)	5548144..5549268	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
ITG66_RS25790 (ITG66_25795)	5549268..5550977	+	1710	WP_003099757.1	PilN family type IVB pilus formation outer membrane protein	-
ITG66_RS25795 (ITG66_25800)	5550981..5552306	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
ITG66_RS25800 (ITG66_25805)	5552296..5552829	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5519327..5606539	87212

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T181158 WP_003120987.1 NZ_CP064393:c5547942-5547643 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T181158 NZ_CP085807:c4362665-4362174 [Salmonella enterica subsp. enterica serovar Typhi]
ATGATCTCCACCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATAGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGCGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCTCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGGTTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTCGCTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGTGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTATCAGTGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT181158 WP_003448662.1 NZ_CP064393:c5547646-5547356 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT181158 NZ_CP085807:c4362955-4362662 [Salmonella enterica subsp. enterica serovar Typhi]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGTGAAACTCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATTGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGTTGGAAGCTGCCCGTGCTGCCGCAGAAGAAGTGCTTA
TCGAACAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGAATTTCTCGTTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Structures

Toxin

• AlphaFold DB: G8CP73