Detailed information of TA system TA181157

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4690667..4691307
• Replicon: chromosome
• Accession: NZ_CP064393
• Organism: Pseudomonas aeruginosa strain SRRSH1101

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ITG66_RS21785
• Protein ID: WP_003134109.1
• Coordinates: 4690667..4691077 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: ITG66_RS21790
• Protein ID: WP_031634724.1
• Coordinates: 4691077..4691307 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ITG66_RS21740 (ITG66_21745)	4685703..4687028	+	1326	WP_191519258.1	STY4528 family pathogenicity island replication protein	-
ITG66_RS31930	4687025..4687537	+	513	WP_223697281.1	hypothetical protein	-
ITG66_RS21755 (ITG66_21760)	4687694..4688422	+	729	WP_009315862.1	TIGR03761 family integrating conjugative element protein	-
ITG66_RS21760 (ITG66_21765)	4688428..4688958	+	531	WP_211851974.1	DUF3158 family protein	-
ITG66_RS21765 (ITG66_21770)	4688972..4689460	+	489	WP_023104904.1	single-stranded DNA-binding protein	-
ITG66_RS21770 (ITG66_21775)	4689959..4690156	+	198	WP_191519259.1	CrpP family ICE-associated protein	-
ITG66_RS21775 (ITG66_21780)	4690222..4690440	-	219	WP_023083218.1	hypothetical protein	-
ITG66_RS21780 (ITG66_21785)	4690490..4690651	-	162	WP_191519260.1	hypothetical protein	-
ITG66_RS21785 (ITG66_21790)	4690667..4691077	-	411	WP_003134109.1	tRNA(fMet)-specific endonuclease VapC	Toxin
ITG66_RS21790 (ITG66_21795)	4691077..4691307	-	231	WP_031634724.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
ITG66_RS21795 (ITG66_21800)	4691625..4693550	+	1926	WP_191519261.1	type I DNA topoisomerase	-
ITG66_RS21800 (ITG66_21805)	4693564..4694310	+	747	WP_223723588.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	katA	4672204..4799052	126848

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-33)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15452.78 Da
• Isoelectric Point: 7.3233

>T181157 WP_003134109.1 NZ_CP064393:c4691077-4690667 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVTPIV

Nucleotide

• Length: 411 bp

>T181157 NZ_CP085807:c4238936-4238448 [Salmonella enterica subsp. enterica serovar Typhi]
ATGATCTCCGCCCCTGAACCGCTTCATGCCGGGCATATTCTTACCCCCTTCTGCTGCGGTGTGGATTCAATGGATAACTG
GCTAAAACAGCGGGCGATGAAAAATCAGGTAACTGGCGCATCCCGTACCTTTGTGTGTTGCGGCAGCGATTCTGACGTAC
TGGCGTATTATTCGCTGGCGTCCAGCGCGGTAACGACAAACACCGCCCCCGGTCGCTTTCGTCGCAATATGCCCGATCCG
ATCCCGGTTGTGGTGCTGGGCCGTCTGGCGGTGGATCGGTCGCTACATGGGCAGGGCGTAGGTCGGGCGCTGGTGAGGGA
TGCCGGGCTGCGGGTGATTCAGGTGGCGGAAACTATCGGTATTCGCGGGATGCTGGTCCATGCCCTGTCGGATGAAGCGC
GGGAGTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTT
CATGCCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8420.29 Da
• Isoelectric Point: 4.2821

>AT181157 WP_031634724.1 NZ_CP064393:c4691307-4691077 [Pseudomonas aeruginosa]
VEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVAVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT181157 NZ_CP085807:c4239226-4238933 [Salmonella enterica subsp. enterica serovar Typhi]
ATGCCAGTCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGCATCAAGCCCGCCGAGCGCGATCTTATCGA
TCGGGCTGCAAAAGCCAGAGGGAAAAACTGCACAGACTTTGTGCTAGAAGCTGCCCGCGCCGCCGCCGAGGAAGCGCTGA
TCGAGCAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGCATTTCTGATCCGTCTGGATCAGGCTCCTGCGCCAAAC
GATGCGTTGCGTAAAACCATGCAGACTCCTGCACCGTGGGAGCAGGAAAAATGA