Detailed information of TA system TA181156

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4614922..4616180
• Replicon: chromosome
• Accession: NZ_CP064393
• Organism: Pseudomonas aeruginosa strain SRRSH1101

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9I4U4
• Locus tag: ITG66_RS21375
• Protein ID: WP_003112528.1
• Coordinates: 4614922..4615677 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: ITG66_RS21380
• Protein ID: WP_003086329.1
• Coordinates: 4615812..4616180 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ITG66_RS21365 (ITG66_21370)	4610095..4612638	+	2544	WP_031755663.1	acyl-homoserine-lactone acylase QuiP	-
ITG66_RS21370 (ITG66_21375)	4612793..4614154	-	1362	WP_073647918.1	DNA recombination protein RmuC	-
ITG66_RS21375 (ITG66_21380)	4614922..4615677	-	756	WP_003112528.1	RES family NAD+ phosphorylase	Toxin
ITG66_RS21380 (ITG66_21385)	4615812..4616180	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
ITG66_RS21385 (ITG66_21390)	4616253..4617539	-	1287	WP_003163134.1	FAD-binding oxidoreductase	-
ITG66_RS21390 (ITG66_21395)	4617595..4619184	-	1590	WP_074231416.1	aldehyde dehydrogenase family protein	-
ITG66_RS21395 (ITG66_21400)	4619263..4619739	+	477	WP_003112531.1	hypothetical protein	-
ITG66_RS21400 (ITG66_21405)	4619742..4620017	-	276	WP_169057979.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28278.42 Da
• Isoelectric Point: 7.7294

>T181156 WP_003112528.1 NZ_CP064393:c4615677-4614922 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T181156 NZ_CP085807:c4121612-4121328 [Salmonella enterica subsp. enterica serovar Typhi]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT181156 WP_003086329.1 NZ_CP064393:c4616180-4615812 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT181156 NZ_CP085807:c4121844-4121602 [Salmonella enterica subsp. enterica serovar Typhi]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCACTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: Q9I4U4

Antitoxin

• AlphaFold DB: V6AJU7