Detailed information of TA system TA181148

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 2500920..2501436
• Replicon: chromosome
• Accession: NZ_CP064392
• Organism: Pseudomonas aeruginosa strain SRRSH15

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: ITG70_RS11920
• Protein ID: WP_222829503.1
• Coordinates: 2501155..2501436 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A482UCX2
• Locus tag: ITG70_RS11915
• Protein ID: WP_033942931.1
• Coordinates: 2500920..2501165 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ITG70_RS11895 (ITG70_11900)	2496152..2497768	-	1617	WP_010794484.1	hypothetical protein	-
ITG70_RS11900 (ITG70_11905)	2497779..2498936	-	1158	WP_010794485.1	site-specific integrase	-
ITG70_RS31345 (ITG70_11910)	2499034..2499168	-	135	WP_261990960.1	hypothetical protein	-
ITG70_RS11905 (ITG70_11915)	2499161..2499598	-	438	WP_222829520.1	S24 family peptidase	-
ITG70_RS11910 (ITG70_11920)	2500037..2500735	+	699	WP_222829502.1	inovirus Gp2 family protein	-
ITG70_RS11915 (ITG70_11925)	2500920..2501165	+	246	WP_033942931.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ITG70_RS11920 (ITG70_11930)	2501155..2501436	+	282	WP_222829503.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ITG70_RS11925 (ITG70_11935)	2501505..2501678	-	174	WP_222829504.1	hypothetical protein	-
ITG70_RS11930 (ITG70_11940)	2501689..2502186	-	498	WP_222829505.1	DNA repair protein RadC	-
ITG70_RS11935 (ITG70_11945)	2502158..2503108	-	951	WP_222829506.1	hydrolase or metal-binding protein	-
ITG70_RS11940 (ITG70_11950)	2503201..2504205	-	1005	WP_222829507.1	YqaJ viral recombinase family protein	-
ITG70_RS11945 (ITG70_11955)	2504283..2505251	-	969	WP_222829508.1	DUF932 domain-containing protein	-
ITG70_RS11950 (ITG70_11960)	2505349..2505678	-	330	WP_261990959.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2480731..2508789	28058

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10777.67 Da
• Isoelectric Point: 10.7574

>T181148 WP_222829503.1 NZ_CP064392:2501155-2501436 [Pseudomonas aeruginosa]
MTYKLEFLPSALKEWEKLGHTLREQAKKKLRERLEAPKVQTDALRDLPGHYKIKLRTAGYRLVYRVEDDRVVVVVVAVGK
RERGAAYKAAAKR

Nucleotide

• Length: 282 bp

>T181148 NZ_CP085806:4600829-4601140 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGAGGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8653.98 Da
• Isoelectric Point: 4.1469

>AT181148 WP_033942931.1 NZ_CP064392:2500920-2501165 [Pseudomonas aeruginosa]
MQSVLADVAVSVSELKKNPTGVMAGAGGMPVAVLNHNRVMGYMVPAELYEQMLDRLDDLDLIDVVNARSGEKGVPVNLDD
L

Nucleotide

• Length: 246 bp

>AT181148 NZ_CP085806:4601141-4601431 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A482UCX2