Detailed information of TA system TA181145

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 143196..143701
• Replicon: chromosome
• Accession: NZ_CP064392
• Organism: Pseudomonas aeruginosa strain SRRSH15

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: ITG70_RS00660
• Protein ID: WP_003083773.1
• Coordinates: 143196..143477 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: ITG70_RS00665
• Protein ID: WP_003083775.1
• Coordinates: 143474..143701 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ITG70_RS00635 (ITG70_00635)	138447..139796	+	1350	WP_003142411.1	C4-dicarboxylate transporter DctA	-
ITG70_RS00640 (ITG70_00640)	139845..140531	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
ITG70_RS00645 (ITG70_00645)	140632..141366	+	735	WP_003117208.1	GntR family transcriptional regulator	-
ITG70_RS00650 (ITG70_00650)	141546..141956	+	411	WP_003101225.1	aegerolysin family protein	-
ITG70_RS00655 (ITG70_00655)	141988..142896	-	909	WP_010793581.1	LysR family transcriptional regulator	-
ITG70_RS00660 (ITG70_00660)	143196..143477	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
ITG70_RS00665 (ITG70_00665)	143474..143701	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
ITG70_RS00670 (ITG70_00670)	143877..144497	-	621	WP_003101226.1	hypothetical protein	-
ITG70_RS00675 (ITG70_00675)	144598..145098	+	501	WP_003101228.1	LEA type 2 family protein	-
ITG70_RS00680 (ITG70_00680)	145171..145512	+	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
ITG70_RS00685 (ITG70_00685)	145594..147021	-	1428	WP_003083784.1	GABA permease	-
ITG70_RS00690 (ITG70_00690)	147190..148683	-	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T181145 WP_003083773.1 NZ_CP064392:c143477-143196 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T181145 NZ_CP085806:c4099558-4099190 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGTCGTTCGCTTACGCGCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT181145 WP_003083775.1 NZ_CP064392:c143701-143474 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT181145 NZ_CP085806:c4099776-4099555 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGCGCGGTGACGGCGGGCCCTGTCAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9