Detailed information of TA system TA181142

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 3667347..3667955
• Replicon: chromosome
• Accession: NZ_CP064391
• Organism: Pseudomonas aeruginosa strain PartH-Paeruginosa-RM8376

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: Q9I5J9
• Locus tag: IUJ43_RS16890
• Protein ID: WP_003114156.1
• Coordinates: 3667347..3667694 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: A0A0B0C355
• Locus tag: IUJ43_RS16895
• Protein ID: WP_003114155.1
• Coordinates: 3667704..3667955 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUJ43_RS16860 (IUJ43_16825)	3662766..3662978	+	213	WP_003114160.1	cysteine-rich CWC family protein	-
IUJ43_RS16865 (IUJ43_16830)	3662978..3663670	+	693	WP_003114159.1	16S rRNA pseudouridine(516) synthase	-
IUJ43_RS16870 (IUJ43_16835)	3663806..3664849	+	1044	WP_003098363.1	L,D-transpeptidase	-
IUJ43_RS16875 (IUJ43_16840)	3664929..3665666	+	738	WP_003114158.1	murein L,D-transpeptidase catalytic domain family protein	-
IUJ43_RS16880 (IUJ43_16845)	3666118..3667020	+	903	WP_003114157.1	(R)-3-hydroxydecanoyl-ACP:CoA transacylase	-
IUJ43_RS16890 (IUJ43_16855)	3667347..3667694	-	348	WP_003114156.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IUJ43_RS16895 (IUJ43_16860)	3667704..3667955	-	252	WP_003114155.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
IUJ43_RS16900 (IUJ43_16865)	3668169..3669152	-	984	WP_003114154.1	tyrosine-type recombinase/integrase	-
IUJ43_RS16905 (IUJ43_16870)	3669152..3670444	-	1293	WP_003115206.1	hypothetical protein	-
IUJ43_RS16910 (IUJ43_16875)	3670674..3671948	-	1275	WP_010895520.1	zonular occludens toxin family protein	-
IUJ43_RS16915 (IUJ43_16880)	3671952..3672308	-	357	WP_003114150.1	DUF2523 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	catB7	-	3660385..3689524	29139

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12984.78 Da
• Isoelectric Point: 4.4212

>T181142 WP_003114156.1 NZ_CP064391:c3667694-3667347 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEVAVILVLRQKQSVEQQLIRYCLVGPIE

Nucleotide

• Length: 348 bp

>T181142 NZ_CP085806:c3573908-3573510 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9442.79 Da
• Isoelectric Point: 9.4808

>AT181142 WP_003114155.1 NZ_CP064391:c3667955-3667704 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Nucleotide

• Length: 252 bp

>AT181142 NZ_CP085806:c3574135-3573908 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Structures

Toxin

• AlphaFold DB: Q9I5J9

Antitoxin

• AlphaFold DB: A0A0B0C355