Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 25880..26439 | Replicon | plasmid unnamed |
| Accession | NZ_CP064388 | ||
| Organism | Escherichia coli O104:H4 strain PartB-EcoliO104H4-RM8376 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | G4VUF8 |
| Locus tag | IUJ37_RS26305 | Protein ID | WP_000421273.1 |
| Coordinates | 25880..26155 (-) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | V0SRR3 |
| Locus tag | IUJ37_RS26310 | Protein ID | WP_001387467.1 |
| Coordinates | 26155..26439 (-) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| IUJ37_RS26265 (IUJ37_26045) | 20922..21152 | + | 231 | WP_001261288.1 | type II toxin-antitoxin system VapB family antitoxin | - |
| IUJ37_RS26270 (IUJ37_26050) | 21176..21577 | + | 402 | WP_001392130.1 | type II toxin-antitoxin system VapC family toxin | - |
| IUJ37_RS26275 (IUJ37_26055) | 21660..21815 | - | 156 | WP_000332512.1 | transposase domain-containing protein | - |
| IUJ37_RS26280 (IUJ37_26060) | 22901..23878 | + | 978 | WP_001387465.1 | RepB family plasmid replication initiator protein | - |
| IUJ37_RS26285 (IUJ37_26065) | 24157..24897 | - | 741 | WP_001066951.1 | tyrosine-type recombinase/integrase | - |
| IUJ37_RS26290 (IUJ37_26070) | 25018..25173 | - | 156 | WP_044713270.1 | hypothetical protein | - |
| IUJ37_RS26295 | 25266..25556 | - | 291 | Protein_37 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| IUJ37_RS26300 (IUJ37_26080) | 25543..25811 | - | 269 | Protein_38 | type II toxin-antitoxin system ParD family antitoxin | - |
| IUJ37_RS26305 (IUJ37_26085) | 25880..26155 | - | 276 | WP_000421273.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| IUJ37_RS26310 (IUJ37_26090) | 26155..26439 | - | 285 | WP_001387467.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| IUJ37_RS26315 (IUJ37_26095) | 27347..28175 | - | 829 | Protein_41 | incFII family plasmid replication initiator RepA | - |
| IUJ37_RS26320 (IUJ37_26100) | 28168..28242 | - | 75 | WP_014966221.1 | RepA leader peptide Tap | - |
| IUJ37_RS26330 (IUJ37_26110) | 28480..28734 | - | 255 | WP_000083850.1 | replication regulatory protein RepA | - |
| IUJ37_RS26335 (IUJ37_26115) | 29015..29995 | + | 981 | WP_000019427.1 | IS5-like element ISKpn26 family transposase | - |
| IUJ37_RS26340 (IUJ37_26120) | 30446..30658 | - | 213 | Protein_45 | ANR family transcriptional regulator | - |
| IUJ37_RS26345 (IUJ37_26125) | 30723..31400 | + | 678 | WP_001339397.1 | IS66-like element accessory protein TnpA | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | aggA / aggB / aggC / aggD / aap/aspU | 1..75554 | 75554 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10765.45 Da Isoelectric Point: 9.9324
>T181116 WP_000421273.1 NZ_CP064388:c26155-25880 [Escherichia coli O104:H4]
MELKWTSKALSDLSRLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR
MELKWTSKALSDLSRLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T181116 NZ_CP085804:c4366141-4365656 [Salmonella enterica subsp. enterica serovar Enteritidis]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGTGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGTGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG
Antitoxin
Download Length: 95 a.a. Molecular weight: 10460.87 Da Isoelectric Point: 5.6558
>AT181116 WP_001387467.1 NZ_CP064388:c26439-26155 [Escherichia coli O104:H4]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR
Download Length: 285 bp
>AT181116 NZ_CP085804:c4366416-4366129 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCAGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCAGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0Y6H1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0Y880 |