Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 20922..21577 | Replicon | plasmid unnamed |
| Accession | NZ_CP064388 | ||
| Organism | Escherichia coli O104:H4 strain PartB-EcoliO104H4-RM8376 | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | A0A2H3M3N3 |
| Locus tag | IUJ37_RS26270 | Protein ID | WP_001392130.1 |
| Coordinates | 21176..21577 (+) | Length | 134 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | A0A0E0YAG4 |
| Locus tag | IUJ37_RS26265 | Protein ID | WP_001261288.1 |
| Coordinates | 20922..21152 (+) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| IUJ37_RS26230 (IUJ37_26010) | 15974..16390 | - | 417 | WP_001278815.1 | plasmid partitioning/stability family protein | - |
| IUJ37_RS26235 (IUJ37_26015) | 16383..17363 | - | 981 | WP_000688504.1 | plasmid segregation protein ParM | - |
| IUJ37_RS26240 (IUJ37_26020) | 17777..18085 | - | 309 | WP_000030204.1 | molecular chaperone GroEL | - |
| IUJ37_RS26245 (IUJ37_26025) | 18172..18816 | - | 645 | WP_001144032.1 | ParA family protein | - |
| IUJ37_RS26250 (IUJ37_26030) | 18995..19801 | - | 807 | WP_000016961.1 | site-specific integrase | - |
| IUJ37_RS26255 (IUJ37_26035) | 19802..20107 | - | 306 | WP_001159872.1 | type II toxin-antitoxin system toxin CcdB | - |
| IUJ37_RS26260 (IUJ37_26040) | 20109..20327 | - | 219 | WP_000813638.1 | type II toxin-antitoxin system antitoxin CcdA | - |
| IUJ37_RS26265 (IUJ37_26045) | 20922..21152 | + | 231 | WP_001261288.1 | type II toxin-antitoxin system VapB family antitoxin | Antitoxin |
| IUJ37_RS26270 (IUJ37_26050) | 21176..21577 | + | 402 | WP_001392130.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| IUJ37_RS26275 (IUJ37_26055) | 21660..21815 | - | 156 | WP_000332512.1 | transposase domain-containing protein | - |
| IUJ37_RS26280 (IUJ37_26060) | 22901..23878 | + | 978 | WP_001387465.1 | RepB family plasmid replication initiator protein | - |
| IUJ37_RS26285 (IUJ37_26065) | 24157..24897 | - | 741 | WP_001066951.1 | tyrosine-type recombinase/integrase | - |
| IUJ37_RS26290 (IUJ37_26070) | 25018..25173 | - | 156 | WP_044713270.1 | hypothetical protein | - |
| IUJ37_RS26295 | 25266..25556 | - | 291 | Protein_37 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| IUJ37_RS26300 (IUJ37_26080) | 25543..25811 | - | 269 | Protein_38 | type II toxin-antitoxin system ParD family antitoxin | - |
| IUJ37_RS26305 (IUJ37_26085) | 25880..26155 | - | 276 | WP_000421273.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| IUJ37_RS26310 (IUJ37_26090) | 26155..26439 | - | 285 | WP_001387467.1 | ribbon-helix-helix domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | aggA / aggB / aggC / aggD / aap/aspU | 1..75554 | 75554 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 134 a.a. Molecular weight: 14647.00 Da Isoelectric Point: 6.7123
>T181115 WP_001392130.1 NZ_CP064388:21176-21577 [Escherichia coli O104:H4]
MLDTNICSFIMREQPAAVIKRLEQAVLRNHRIVVSAITYAEMRFDATGPKASPRHIALVDAFCARLDAILPWDRAAVDAT
TEIKMALRLAETPIGPNNTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK
MLDTNICSFIMREQPAAVIKRLEQAVLRNHRIVVSAITYAEMRFDATGPKASPRHIALVDAFCARLDAILPWDRAAVDAT
TEIKMALRLAETPIGPNNTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK
Download Length: 402 bp
>T181115 NZ_CP085804:c4256069-4255701 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGTCGTTCGCTTACGCGCCTGA
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGTCGTTCGCTTACGCGCCTGA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8684.76 Da Isoelectric Point: 4.3654
>AT181115 WP_001261288.1 NZ_CP064388:20922-21152 [Escherichia coli O104:H4]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWSSFAALEKADADFMAEREDIVSDEGRFDL
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWSSFAALEKADADFMAEREDIVSDEGRFDL
Download Length: 231 bp
>AT181115 NZ_CP085804:c4256287-4256066 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGCGCGGTGACGGCGGGCCCTGTCAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGCGCGGTGACGGCGGGCCCTGTCAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2H3M3N3 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0YAG4 |