Detailed information of TA system TA181114

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 19802..20327
• Replicon: plasmid unnamed
• Accession: NZ_CP064388
• Organism: Escherichia coli O104:H4 strain PartB-EcoliO104H4-RM8376

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A0E0Y871
• Locus tag: IUJ37_RS26255
• Protein ID: WP_001159872.1
• Coordinates: 19802..20107 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A0E0Y6P9
• Locus tag: IUJ37_RS26260
• Protein ID: WP_000813638.1
• Coordinates: 20109..20327 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUJ37_RS26230 (IUJ37_26010)	15974..16390	-	417	WP_001278815.1	plasmid partitioning/stability family protein	-
IUJ37_RS26235 (IUJ37_26015)	16383..17363	-	981	WP_000688504.1	plasmid segregation protein ParM	-
IUJ37_RS26240 (IUJ37_26020)	17777..18085	-	309	WP_000030204.1	molecular chaperone GroEL	-
IUJ37_RS26245 (IUJ37_26025)	18172..18816	-	645	WP_001144032.1	ParA family protein	-
IUJ37_RS26250 (IUJ37_26030)	18995..19801	-	807	WP_000016961.1	site-specific integrase	-
IUJ37_RS26255 (IUJ37_26035)	19802..20107	-	306	WP_001159872.1	type II toxin-antitoxin system toxin CcdB	Toxin
IUJ37_RS26260 (IUJ37_26040)	20109..20327	-	219	WP_000813638.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
IUJ37_RS26265 (IUJ37_26045)	20922..21152	+	231	WP_001261288.1	type II toxin-antitoxin system VapB family antitoxin	-
IUJ37_RS26270 (IUJ37_26050)	21176..21577	+	402	WP_001392130.1	type II toxin-antitoxin system VapC family toxin	-
IUJ37_RS26275 (IUJ37_26055)	21660..21815	-	156	WP_000332512.1	transposase domain-containing protein	-
IUJ37_RS26280 (IUJ37_26060)	22901..23878	+	978	WP_001387465.1	RepB family plasmid replication initiator protein	-
IUJ37_RS26285 (IUJ37_26065)	24157..24897	-	741	WP_001066951.1	tyrosine-type recombinase/integrase	-
IUJ37_RS26290 (IUJ37_26070)	25018..25173	-	156	WP_044713270.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	aggA / aggB / aggC / aggD / aap/aspU	1..75554	75554

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11722.51 Da
• Isoelectric Point: 5.6919

>T181114 WP_001159872.1 NZ_CP064388:c20107-19802 [Escherichia coli O104:H4]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHWENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T181114 NZ_CP085804:c4207650-4207375 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGAGCCTGACTGGATTCTTATTTATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8344.29 Da
• Isoelectric Point: 4.5203

>AT181114 WP_000813638.1 NZ_CP064388:c20327-20109 [Escherichia coli O104:H4]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVAKFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT181114 NZ_CP085804:c4207913-4207653 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGCTGCAAATGCGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGGACCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGCATAC
CCAATGAGTTAACGTCACGAACGATAGAAAATAGCGAAGCTGGCGTTGATATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y871

Antitoxin

• AlphaFold DB: A0A0E0Y6P9