Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 5054148..5054773 Replicon chromosome
Accession NZ_CP064387
Organism Escherichia coli O104:H4 strain PartB-EcoliO104H4-RM8376

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag IUJ37_RS24860 Protein ID WP_000911329.1
Coordinates 5054375..5054773 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag IUJ37_RS24855 Protein ID WP_000450524.1
Coordinates 5054148..5054375 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IUJ37_RS24830 (5049950) 5049950..5050420 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
IUJ37_RS24835 (5050420) 5050420..5050992 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
IUJ37_RS24840 (5051138) 5051138..5052016 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
IUJ37_RS24845 (5052033) 5052033..5053067 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
IUJ37_RS24850 (5053280) 5053280..5053993 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
IUJ37_RS24855 (5054148) 5054148..5054375 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
IUJ37_RS24860 (5054375) 5054375..5054773 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
IUJ37_RS24865 (5054920) 5054920..5055783 + 864 WP_001267498.1 neutral zinc metallopeptidase -
IUJ37_RS24870 (5055798) 5055798..5057813 + 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
IUJ37_RS24875 (5057887) 5057887..5058585 + 699 WP_000679823.1 esterase -
IUJ37_RS24880 (5058695) 5058695..5058895 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T181113 WP_000911329.1 NZ_CP064387:5054375-5054773 [Escherichia coli O104:H4]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T181113 NZ_CP085804:c3741323-3740910 [Salmonella enterica subsp. enterica serovar Enteritidis]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT181113 WP_000450524.1 NZ_CP064387:5054148-5054375 [Escherichia coli O104:H4]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT181113 NZ_CP085804:c3741570-3741304 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGACGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References