Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4609269..4609923 Replicon chromosome
Accession NZ_CP064387
Organism Escherichia coli O104:H4 strain PartB-EcoliO104H4-RM8376

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag IUJ37_RS22750 Protein ID WP_000244772.1
Coordinates 4609516..4609923 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IUJ37_RS22745 Protein ID WP_000354046.1
Coordinates 4609269..4609535 (+) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IUJ37_RS22720 (4604556) 4604556..4605299 + 744 WP_000951964.1 SDR family oxidoreductase -
IUJ37_RS22725 (4605356) 4605356..4606789 - 1434 WP_001387034.1 6-phospho-beta-glucosidase BglA -
IUJ37_RS22730 (4606834) 4606834..4607145 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
IUJ37_RS22735 (4607309) 4607309..4607968 + 660 WP_000250274.1 hemolysin III family protein -
IUJ37_RS22740 (4608046) 4608046..4609026 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
IUJ37_RS22745 (4609269) 4609269..4609535 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IUJ37_RS22750 (4609516) 4609516..4609923 + 408 WP_000244772.1 protein YgfX Toxin
IUJ37_RS22755 (4609963) 4609963..4610484 - 522 WP_001055874.1 flavodoxin FldB -
IUJ37_RS22760 (4610596) 4610596..4611492 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
IUJ37_RS22765 (4611517) 4611517..4612227 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IUJ37_RS22770 (4612233) 4612233..4613966 + 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-131)

Antitoxin

(7-79)


Sequences


Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T181111 WP_000244772.1 NZ_CP064387:4609516-4609923 [Escherichia coli O104:H4]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T181111 NZ_CP085804:3577556-3578083 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTACTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT181111 WP_000354046.1 NZ_CP064387:4609269-4609535 [Escherichia coli O104:H4]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT181111 NZ_CP085804:3577269-3577559 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTGCGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGCGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4


Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References