Detailed information of TA system TA181110

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4449829..4450630
• Replicon: chromosome
• Accession: NZ_CP064387
• Organism: Escherichia coli O104:H4 strain PartB-EcoliO104H4-RM8376

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: D3GU15
• Locus tag: IUJ37_RS21940
• Protein ID: WP_001094429.1
• Coordinates: 4449829..4450206 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0E0XUQ1
• Locus tag: IUJ37_RS21945
• Protein ID: WP_001285616.1
• Coordinates: 4450253..4450630 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUJ37_RS21905 (4444910)	4444910..4445509	+	600	WP_001255040.1	type II secretion system minor pseudopilin GspJ	-
IUJ37_RS21910 (4445512)	4445512..4446489	+	978	WP_000633238.1	type II secretion system minor pseudopilin GspK	-
IUJ37_RS21915 (4446486)	4446486..4447664	+	1179	WP_000094962.1	type II secretion system protein GspL	-
IUJ37_RS21920 (4447666)	4447666..4448202	+	537	WP_000942795.1	GspM family type II secretion system protein YghD	-
IUJ37_RS21925 (4448483)	4448483..4449325	-	843	WP_001280493.1	DUF4942 domain-containing protein	-
IUJ37_RS21930 (4449410)	4449410..4449607	-	198	WP_085949090.1	DUF957 domain-containing protein	-
IUJ37_RS21935 (4449635)	4449635..4449832	-	198	Protein_4290	DUF5983 family protein	-
IUJ37_RS21940 (4449829)	4449829..4450206	-	378	WP_001094429.1	TA system toxin CbtA family protein	Toxin
IUJ37_RS21945 (4450253)	4450253..4450630	-	378	WP_001285616.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
IUJ37_RS21950 (4450710)	4450710..4450931	-	222	WP_000692350.1	DUF987 domain-containing protein	-
IUJ37_RS21955 (4451018)	4451018..4451494	-	477	WP_001388380.1	RadC family protein	-
IUJ37_RS21960 (4451510)	4451510..4451983	-	474	WP_000855081.1	antirestriction protein	-
IUJ37_RS21965 (4452285)	4452285..4453103	-	819	WP_001234603.1	DUF932 domain-containing protein	-
IUJ37_RS21970 (4453203)	4453203..4453436	-	234	WP_001119729.1	DUF905 family protein	-
IUJ37_RS21975 (4453515)	4453515..4453970	-	456	WP_000581502.1	IrmA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	gspC / gspD / gspE / gspF / gspG / gspH / gspI / gspJ / gspK / gspL / gspM / vipA/tssB / vipB/tssC / clbK	4353436..4508881	155445

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13856.73 Da
• Isoelectric Point: 6.8601

>T181110 WP_001094429.1 NZ_CP064387:c4450206-4449829 [Escherichia coli O104:H4]
MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T181110 NZ_CP085804:2091068-2091223 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13747.65 Da
• Isoelectric Point: 6.6240

>AT181110 WP_001285616.1 NZ_CP064387:c4450630-4450253 [Escherichia coli O104:H4]
VSDTLPGTTPPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTMTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPATTV

Nucleotide

• Length: 378 bp

>AT181110 NZ_CP085804:c2091056-2090998 [Salmonella enterica subsp. enterica serovar Enteritidis]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGCAAAGCATGAAATTGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A0E0XVU5

Antitoxin

• AlphaFold DB: A0A0E0XUQ1