Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3937380..3937966 Replicon chromosome
Accession NZ_CP064387
Organism Escherichia coli O104:H4 strain PartB-EcoliO104H4-RM8376

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag IUJ37_RS19360 Protein ID WP_042014162.1
Coordinates 3937380..3937610 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag IUJ37_RS19365 Protein ID WP_000593555.1
Coordinates 3937607..3937966 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IUJ37_RS19350 (3933417) 3933417..3936152 + 2736 WP_000149085.1 ribosome-associated ATPase/putative transporter RbbA -
IUJ37_RS19355 (3936152) 3936152..3937276 + 1125 WP_001216257.1 ABC transporter permease -
IUJ37_RS19360 (3937380) 3937380..3937610 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
IUJ37_RS19365 (3937607) 3937607..3937966 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
IUJ37_RS19370 (3938086) 3938086..3938487 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
IUJ37_RS19375 (3938493) 3938493..3939299 - 807 WP_000173630.1 nickel import ATP-binding protein NikE -
IUJ37_RS19380 (3939296) 3939296..3940060 - 765 WP_001136229.1 nickel import ATP-binding protein NikD -
IUJ37_RS19385 (3940060) 3940060..3940893 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
IUJ37_RS19390 (3940890) 3940890..3941834 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T181108 WP_042014162.1 NZ_CP064387:3937380-3937610 [Escherichia coli O104:H4]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T181108 NZ_CP085804:c1123094-1122876 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT181108 WP_000593555.1 NZ_CP064387:3937607-3937966 [Escherichia coli O104:H4]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT181108 NZ_CP085804:c1123496-1123122 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References