Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3128932..3129429 Replicon chromosome
Accession NZ_CP064387
Organism Escherichia coli O104:H4 strain PartB-EcoliO104H4-RM8376

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag IUJ37_RS15605 Protein ID WP_001173343.1
Coordinates 3128932..3129105 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag IUJ37_RS15610 Protein ID WP_000398619.1
Coordinates 3129133..3129429 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IUJ37_RS15595 (3125602) 3125602..3127059 + 1458 WP_000856837.1 dipeptide/tripeptide permease DtpC -
IUJ37_RS15600 (3127296) 3127296..3128813 + 1518 WP_001295074.1 lysine--tRNA ligase -
IUJ37_RS15605 (3128932) 3128932..3129105 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
IUJ37_RS15610 (3129133) 3129133..3129429 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
IUJ37_RS15615 (3129656) 3129656..3129928 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
IUJ37_RS15620 (3129940) 3129940..3130170 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
IUJ37_RS15625 (3130351) 3130351..3131982 + 1632 WP_001216486.1 sensor histidine kinase -
IUJ37_RS15630 (3131979) 3131979..3132698 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T181105 WP_001173343.1 NZ_CP064387:c3129105-3128932 [Escherichia coli O104:H4]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T181105 NZ_CP085804:503397-503705 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCAATACATGGTTTATCGCAATAAAGGAAACAGCAAGGCTTATCCTTATTTACTTGATGTGCAAAGCGATATTATTGA
TGAACTGCATACTCGCATGGTTATCCCATTGTTTCCTGTGAGCAGATTGGTAAATAACCCAGTTAAGCGCCTCACGCCCA
CTCTGAATGTGGAAGGTAATGACTATCTGGTGATGACTCACGAAATGGCGAGTATCAGGCTTTCGCAGATTGGTGATGAG
GTGATGGACGTTCGTTCCCACAGGCAAACCATCAAAAATGCTCTCGATTTTATCTTTGACGGGTTCTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT181105 WP_000398619.1 NZ_CP064387:c3129429-3129133 [Escherichia coli O104:H4]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT181105 NZ_CP085804:503155-503394 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCCGGGTATGAGTGTACGACATAAAAAAACAGTCAGTGTCACGCTGGAACCTGCATTGCTTCAGCAAGCCCGTGATGC
AGGTATTAATCTGTCAGCGGTGCTTACCGCCGCGCTGAAAGAGGAAATCAGTGCCACAGGCGTTGAACGCTGGAAAGCAG
AAAATAGAGCGGGTTTGCAGGAGCTCAATCGTATTACTGATGAGCACGGCCTTTTATCGGACGATTACAGGACGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References