Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 2981386..2981981 Replicon chromosome
Accession NZ_CP064387
Organism Escherichia coli O104:H4 strain PartB-EcoliO104H4-RM8376

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag IUJ37_RS14865 Protein ID WP_000239579.1
Coordinates 2981386..2981736 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag IUJ37_RS14870 Protein ID WP_001223208.1
Coordinates 2981730..2981981 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IUJ37_RS14845 (2976662) 2976662..2977684 - 1023 WP_001387274.1 ABC transporter permease -
IUJ37_RS14850 (2977698) 2977698..2979200 - 1503 WP_000205794.1 sugar ABC transporter ATP-binding protein -
IUJ37_RS14855 (2979510) 2979510..2980466 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
IUJ37_RS14860 (2980776) 2980776..2981306 + 531 WP_000055072.1 inorganic diphosphatase -
IUJ37_RS14865 (2981386) 2981386..2981736 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
IUJ37_RS14870 (2981730) 2981730..2981981 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
IUJ37_RS14875 (2982193) 2982193..2982534 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
IUJ37_RS14880 (2982537) 2982537..2986316 - 3780 WP_000060946.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T181103 WP_000239579.1 NZ_CP064387:c2981736-2981386 [Escherichia coli O104:H4]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T181103 NZ_CP085804:355193-355684 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGATCTCCACCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATAGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGCGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCTCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGGTTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTCGCTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGTGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTATCAGCGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT181103 WP_001223208.1 NZ_CP064387:c2981981-2981730 [Escherichia coli O104:H4]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT181103 NZ_CP085804:354903-355196 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATTGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACTGACTTTGTGCTGGAAGCTGCCCGTGCTGCCGCCGAGGAAGCGCTGA
TCGAACAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGAGTTTCTCGTTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References