Detailed information of TA system TA181101

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 2314659..2315496
• Replicon: chromosome
• Accession: NZ_CP064387
• Organism: Escherichia coli O104:H4 strain PartB-EcoliO104H4-RM8376

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: IUJ37_RS11695
• Protein ID: WP_000227784.1
• Coordinates: 2314954..2315496 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: IUJ37_RS11690
• Protein ID: WP_001297137.1
• Coordinates: 2314659..2314970 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUJ37_RS11665 (2309679)	2309679..2310626	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
IUJ37_RS11670 (2310648)	2310648..2312639	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
IUJ37_RS11675 (2312629)	2312629..2313243	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
IUJ37_RS11680 (2313243)	2313243..2313572	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
IUJ37_RS11685 (2313584)	2313584..2314474	+	891	WP_000971336.1	heme o synthase	-
IUJ37_RS11690 (2314659)	2314659..2314970	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
IUJ37_RS11695 (2314954)	2314954..2315496	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
IUJ37_RS11700 (2315552)	2315552..2316487	-	936	WP_024174603.1	tetratricopeptide repeat protein	-
IUJ37_RS11705 (2316895)	2316895..2318259	+	1365	WP_001000974.1	MFS transporter	-
IUJ37_RS11710 (2318387)	2318387..2318878	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
IUJ37_RS11715 (2319046)	2319046..2319957	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T181101 WP_000227784.1 NZ_CP064387:2314954-2315496 [Escherichia coli O104:H4]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T181101 NZ_CP085804:c44614-44303 [Salmonella enterica subsp. enterica serovar Enteritidis]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACACTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGCACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATGATCGGGGTAATAAAGAATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT181101 WP_001297137.1 NZ_CP064387:2314659-2314970 [Escherichia coli O104:H4]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT181101 NZ_CP085804:c44306-43860 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAAGCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTCAAACTACCAGCAGATGCCTTCATCGAGTAG

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3