Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2280582..2281200 Replicon chromosome
Accession NZ_CP064387
Organism Escherichia coli O104:H4 strain PartB-EcoliO104H4-RM8376

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag IUJ37_RS11525 Protein ID WP_001291435.1
Coordinates 2280982..2281200 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag IUJ37_RS11520 Protein ID WP_000344800.1
Coordinates 2280582..2280956 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IUJ37_RS11510 (2275671) 2275671..2276864 + 1194 WP_001386618.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
IUJ37_RS11515 (2276887) 2276887..2280036 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
IUJ37_RS11520 (2280582) 2280582..2280956 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
IUJ37_RS11525 (2280982) 2280982..2281200 + 219 WP_001291435.1 HHA domain-containing protein Toxin
IUJ37_RS11530 (2281371) 2281371..2281922 + 552 WP_000102564.1 maltose O-acetyltransferase -
IUJ37_RS11535 (2282038) 2282038..2282508 + 471 WP_000136192.1 YlaC family protein -
IUJ37_RS11540 (2282672) 2282672..2284222 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
IUJ37_RS11545 (2284264) 2284264..2284617 - 354 WP_000878140.1 DUF1428 domain-containing protein -
IUJ37_RS11555 (2284996) 2284996..2285307 + 312 WP_000409911.1 MGMT family protein -
IUJ37_RS11560 (2285338) 2285338..2285910 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T181100 WP_001291435.1 NZ_CP064387:2280982-2281200 [Escherichia coli O104:H4]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T181100 NZ_CP085803:c4460544-4460059 [Salmonella enterica subsp. enterica serovar Enteritidis]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGTGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT181100 WP_000344800.1 NZ_CP064387:2280582-2280956 [Escherichia coli O104:H4]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT181100 NZ_CP085803:c4460819-4460532 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCAGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References