Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 79374..79933 Replicon plasmid unnamed
Accession NZ_CP064384
Organism Escherichia coli O157:H7 strain PartA-EcoliO157H7-RM8376

Toxin (Protein)


Gene name relE Uniprot ID O82928
Locus tag IUJ36_RS28150 Protein ID WP_000421248.1
Coordinates 79658..79933 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag IUJ36_RS28145 Protein ID WP_001178089.1
Coordinates 79374..79658 (+) Length 95 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IUJ36_RS28105 (74565) 74565..75125 + 561 WP_000840472.1 fertility inhibition protein FinO -
IUJ36_RS28110 (75258) 75258..75470 + 213 WP_001302189.1 ANR family transcriptional regulator -
IUJ36_RS28115 (75715) 75715..76176 + 462 WP_001233853.1 thermonuclease family protein -
IUJ36_RS28120 (76222) 76222..76431 + 210 WP_001302200.1 hemolysin expression modulator Hha -
IUJ36_RS28125 (76469) 76469..76807 + 339 WP_000766796.1 hypothetical protein -
IUJ36_RS28130 (77047) 77047..77301 + 255 WP_000083831.1 replication regulatory protein RepA -
IUJ36_RS28135 (77537) 77537..77611 + 75 WP_001370046.1 RepA leader peptide Tap -
IUJ36_RS28140 (77604) 77604..78461 + 858 WP_000130945.1 incFII family plasmid replication initiator RepA -
IUJ36_RS28145 (79374) 79374..79658 + 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
IUJ36_RS28150 (79658) 79658..79933 + 276 WP_000421248.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IUJ36_RS28155 (80028) 80028..80234 + 207 WP_001105064.1 hypothetical protein -
IUJ36_RS28160 (80334) 80334..80549 - 216 Protein_88 transposase -
IUJ36_RS28165 (80593) 80593..81159 - 567 Protein_89 IS3-like element IS600 family transposase -
IUJ36_RS28170 (81159) 81159..81575 - 417 Protein_90 IS91 family transposase -
IUJ36_RS28175 (81575) 81575..81760 - 186 WP_001302179.1 hypothetical protein -
IUJ36_RS28180 (81937) 81937..84147 + 2211 WP_000592771.1 catalase/peroxidase KatP -
IUJ36_RS28185 (84191) 84191..84580 + 390 WP_001172748.1 cytochrome b562 -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB / espP 1..92060 92060


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 92 a.a.        Molecular weight: 10735.42 Da        Isoelectric Point: 9.9324

>T181061 WP_000421248.1 NZ_CP064384:79658-79933 [Escherichia coli O157:H7]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T181061 NZ_CP085801:c3599707-3599399 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCAATACATGGTTTATCGCAATAAAGGAAACAGCAAGGCTTATCCTTATTTACTTGATGTGCAAAGCGATATTATTGA
TGAACTGCATACTCGCATGGTTATCCCATTGTTTCCTGTGAGCAGATTGGTAAATAACCCAGTTAAGCGCCTCACGCCCA
CTCTGAATGTGGAAGGTAATGACTATCTGGTGATGACTCACGAAATGGCGAGTATCAGGCTTTCGCAGATTGGTGATGAG
GTGATGGACGTTCGTTCCCACAGGCAAACCATCAAAAATGCTCTCGATTTTATCTTTGACGGGTTCTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT181061 WP_001178089.1 NZ_CP064384:79374-79658 [Escherichia coli O157:H7]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT181061 NZ_CP085801:c3599949-3599710 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCCGGGTATGAGTGTACGACATAAAAAAACAGTCAGTGTCACGCTGGAACCTGCATTGCTTCAGCAAGCCCGTGATGC
AGGTATTAATCTGTCAGCGGTGCTTACCGCCGCGCTGAAAGAGGAAATCAGTGCCACAGGCGTTGAACGCTGGAAAGCAG
AAAATAGAGCGGGTTTGCAGGAGCTCAATCGTATTACTGATGAGCACGGCCTTTTATCGGACGATTACAGGACGTTTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A2Z6FAZ0


Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References