Detailed information of TA system TA181060

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 34379..34904
• Replicon: plasmid unnamed
• Accession: NZ_CP064384
• Organism: Escherichia coli O157:H7 strain PartA-EcoliO157H7-RM8376

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V0SSI5
• Locus tag: IUJ36_RS27890
• Protein ID: WP_001159868.1
• Coordinates: 34599..34904 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1PPD8
• Locus tag: IUJ36_RS27885
• Protein ID: WP_000813634.1
• Coordinates: 34379..34597 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUJ36_RS27855 (29662)	29662..29892	+	231	WP_010891288.1	hypothetical protein	-
IUJ36_RS27860 (30013)	30013..30753	+	741	WP_001066920.1	tyrosine-type recombinase/integrase	-
IUJ36_RS27865 (31038)	31038..32015	-	978	WP_000361615.1	RepB family plasmid replication initiator protein	-
IUJ36_RS27870 (32423)	32423..32623	-	201	WP_000708307.1	hypothetical protein	-
IUJ36_RS27875 (32620)	32620..33240	-	621	WP_001248529.1	hypothetical protein	-
IUJ36_RS27880 (33237)	33237..33920	-	684	WP_010891291.1	DNA-binding protein	-
IUJ36_RS27885 (34379)	34379..34597	+	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
IUJ36_RS27890 (34599)	34599..34904	+	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	Toxin
IUJ36_RS27895 (34905)	34905..35711	+	807	WP_000016989.1	site-specific integrase	-
IUJ36_RS27905 (36973)	36973..37311	+	339	WP_071525396.1	RepB family plasmid replication initiator protein	-
IUJ36_RS27910 (37899)	37899..39065	+	1167	WP_000772446.1	plasmid-partitioning protein SopA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB / espP	1..92060	92060

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11706.51 Da
• Isoelectric Point: 6.4674

>T181060 WP_001159868.1 NZ_CP064384:34599-34904 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T181060 NZ_CP085801:c3555717-3555430 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGCTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCTCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.34 Da
• Isoelectric Point: 4.7405

>AT181060 WP_000813634.1 NZ_CP064384:34379-34597 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT181060 NZ_CP085801:c3555443-3555141 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAGCATGGTTAAACATAAACGCGGCAATGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Structures

Toxin

• PDB: 4VUB
• PDB: 2VUB
• PDB: 7EPI
• PDB: 7EPG
• PDB: 3VUB
• PDB: 1X75
• PDB: 1VUB
• PDB: 7EPJ
• PDB: 3HPW
• PDB: 3G7Z

Antitoxin

• PDB: 3TCJ
• PDB: 2H3A
• PDB: 2ADN
• PDB: 2ADL
• PDB: 3HPW
• PDB: 2H3C
• PDB: 3G7Z
• AlphaFold DB: A0A829CQY2