Detailed information of TA system TA181058

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 5201430..5201980
• Replicon: chromosome
• Accession: NZ_CP064383
• Organism: Escherichia coli O157:H7 strain PartA-EcoliO157H7-RM8376

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: IUJ36_RS26020
• Protein ID: WP_001160975.1
• Coordinates: 5201666..5201980 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: IUJ36_RS26015
• Protein ID: WP_000125566.1
• Coordinates: 5201430..5201663 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUJ36_RS25990 (5196504)	5196504..5196791	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
IUJ36_RS25995 (5196849)	5196849..5198180	+	1332	WP_001183177.1	MFS transporter	-
IUJ36_RS26000 (5198288)	5198288..5198818	+	531	WP_000600700.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
IUJ36_RS26005 (5198811)	5198811..5200673	+	1863	WP_000377184.1	glutathione-regulated potassium-efflux system protein KefC	-
IUJ36_RS26010 (5200865)	5200865..5201344	+	480	WP_000624372.1	type 3 dihydrofolate reductase	-
IUJ36_RS26015 (5201430)	5201430..5201663	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
IUJ36_RS26020 (5201666)	5201666..5201980	+	315	WP_001160975.1	CcdB family protein	Toxin
IUJ36_RS26025 (5201977)	5201977..5202825	-	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
IUJ36_RS26030 (5202832)	5202832..5203209	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
IUJ36_RS26035 (5203212)	5203212..5204033	-	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
IUJ36_RS26040 (5204030)	5204030..5205019	-	990	WP_000241259.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
IUJ36_RS26045 (5205019)	5205019..5206305	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T181058 WP_001160975.1 NZ_CP064383:5201666-5201980 [Escherichia coli O157:H7]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T181058 NZ_CP085801:2980013-2980231 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT181058 WP_000125566.1 NZ_CP064383:5201430-5201663 [Escherichia coli O157:H7]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT181058 NZ_CP085801:2979611-2979985 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9