Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4855204..4855701 Replicon chromosome
Accession NZ_CP064383
Organism Escherichia coli O157:H7 strain PartA-EcoliO157H7-RM8376

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag IUJ36_RS24470 Protein ID WP_001173343.1
Coordinates 4855528..4855701 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag IUJ36_RS24465 Protein ID WP_000398619.1
Coordinates 4855204..4855500 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IUJ36_RS24445 (4851935) 4851935..4852654 - 720 WP_000611288.1 two-component system response regulator DcuR -
IUJ36_RS24450 (4852651) 4852651..4854282 - 1632 WP_001216477.1 sensor histidine kinase -
IUJ36_RS24455 (4854463) 4854463..4854693 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
IUJ36_RS24460 (4854705) 4854705..4854977 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
IUJ36_RS24465 (4855204) 4855204..4855500 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
IUJ36_RS24470 (4855528) 4855528..4855701 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
IUJ36_RS24475 (4855820) 4855820..4857337 - 1518 WP_001295074.1 lysine--tRNA ligase -
IUJ36_RS24480 (4857574) 4857574..4859031 - 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T181056 WP_001173343.1 NZ_CP064383:4855528-4855701 [Escherichia coli O157:H7]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T181056 NZ_CP085801:c2012039-2011884 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT181056 WP_000398619.1 NZ_CP064383:4855204-4855500 [Escherichia coli O157:H7]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT181056 NZ_CP085801:2012051-2012109 [Salmonella enterica subsp. enterica serovar Enteritidis]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGCAAAGCATGAAATTGGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References