Detailed information of TA system TA181051

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 3954896..3955650
• Replicon: chromosome
• Accession: NZ_CP064383
• Organism: Escherichia coli O157:H7 strain PartA-EcoliO157H7-RM8376

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: IUJ36_RS20290
• Protein ID: WP_001301452.1
• Coordinates: 3955165..3955650 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: Q8X700
• Locus tag: IUJ36_RS20285
• Protein ID: WP_000801912.1
• Coordinates: 3954896..3955174 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUJ36_RS20280 (3952124)	3952124..3954829	+	2706	WP_000906970.1	HTH-type transcriptional regulator MalT	-
IUJ36_RS20285 (3954896)	3954896..3955174	+	279	WP_000801912.1	DUF1778 domain-containing protein	Antitoxin
IUJ36_RS20290 (3955165)	3955165..3955650	+	486	WP_001301452.1	GNAT family N-acetyltransferase	Toxin
IUJ36_RS20295 (3955700)	3955700..3956728	-	1029	WP_000827117.1	RNA 3'-terminal phosphate cyclase	-
IUJ36_RS20300 (3956732)	3956732..3957958	-	1227	WP_001105473.1	RNA-splicing ligase RtcB	-
IUJ36_RS20305 (3958146)	3958146..3959744	+	1599	WP_001232889.1	DNA-binding transcriptional regulator RtcR	-
IUJ36_RS20310 (3959726)	3959726..3960484	-	759	WP_001302007.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-92)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17650.57 Da
• Isoelectric Point: 9.4066

>T181051 WP_001301452.1 NZ_CP064383:3955165-3955650 [Escherichia coli O157:H7]
MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRVYVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAP
ESLPVVVLGRLAIDQAWAGKGLGVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
E

Nucleotide

• Length: 486 bp

>T181051 NZ_CP085800:4382846-4383244 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10420.94 Da
• Isoelectric Point: 4.6336

>AT181051 WP_000801912.1 NZ_CP064383:3954896-3955174 [Escherichia coli O157:H7]
MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACREAQDILLDQRLFILDDEQYDAFLAALDAPITAERQAK
INALMNRKSPWE

Nucleotide

• Length: 279 bp

>AT181051 NZ_CP085800:4382619-4382846 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LHB9