Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3465495..3466149 Replicon chromosome
Accession NZ_CP064383
Organism Escherichia coli O157:H7 strain PartA-EcoliO157H7-RM8376

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag IUJ36_RS17770 Protein ID WP_000244781.1
Coordinates 3465495..3465902 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IUJ36_RS17775 Protein ID WP_000354046.1
Coordinates 3465883..3466149 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IUJ36_RS17750 (3461452) 3461452..3463185 - 1734 WP_000813185.1 single-stranded-DNA-specific exonuclease RecJ -
IUJ36_RS17755 (3463191) 3463191..3463901 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IUJ36_RS17760 (3463926) 3463926..3464822 - 897 WP_000806640.1 site-specific tyrosine recombinase XerD -
IUJ36_RS17765 (3464934) 3464934..3465455 + 522 WP_001055874.1 flavodoxin FldB -
IUJ36_RS17770 (3465495) 3465495..3465902 - 408 WP_000244781.1 protein YgfX Toxin
IUJ36_RS17775 (3465883) 3465883..3466149 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IUJ36_RS17780 (3466392) 3466392..3467372 + 981 WP_000886053.1 tRNA-modifying protein YgfZ -
IUJ36_RS17785 (3467449) 3467449..3468108 - 660 WP_000250274.1 hemolysin III family protein -
IUJ36_RS17790 (3468272) 3468272..3468583 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
IUJ36_RS17795 (3468628) 3468628..3470061 + 1434 WP_001310226.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T181048 WP_000244781.1 NZ_CP064383:c3465902-3465495 [Escherichia coli O157:H7]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T181048 NZ_CP085800:3857206-3857574 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGTCGTTCGCTTACGCGCCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT181048 WP_000354046.1 NZ_CP064383:c3466149-3465883 [Escherichia coli O157:H7]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT181048 NZ_CP085800:3856988-3857209 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGCGCGGTGACGGCGGGCCCTGTCAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References