Detailed information of TA system TA181028

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 4897813..4898855
• Replicon: chromosome
• Accession: NZ_CP064377
• Organism: Achromobacter xylosoxidans strain PartM-Axylosoxidans-RM8376

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: A0A0D6GAF2
• Locus tag: IUJ48_RS22390
• Protein ID: WP_058207229.1
• Coordinates: 4898280..4898855 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A972JB12
• Locus tag: IUJ48_RS22385
• Protein ID: WP_024067903.1
• Coordinates: 4897813..4898283 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUJ48_RS22350 (IUJ48_22300)	4893168..4894589	-	1422	WP_024067909.1	TIGR03752 family integrating conjugative element protein	-
IUJ48_RS22355 (IUJ48_22305)	4894579..4895517	-	939	WP_024067908.1	TIGR03749 family integrating conjugative element protein	-
IUJ48_RS22360 (IUJ48_22310)	4895514..4896212	-	699	WP_024067907.1	TIGR03746 family integrating conjugative element protein	-
IUJ48_RS22365 (IUJ48_22315)	4896209..4896607	-	399	WP_058207559.1	TIGR03750 family conjugal transfer protein	-
IUJ48_RS22370 (IUJ48_22320)	4896621..4896980	-	360	WP_024067905.1	TIGR03745 family integrating conjugative element membrane protein	-
IUJ48_RS22375 (IUJ48_22325)	4896997..4897230	-	234	WP_013520781.1	TIGR03758 family integrating conjugative element protein	-
IUJ48_RS22380 (IUJ48_22330)	4897227..4897610	-	384	WP_024067904.1	RAQPRD family integrative conjugative element protein	-
IUJ48_RS22385 (IUJ48_22335)	4897813..4898283	+	471	WP_024067903.1	helix-turn-helix domain-containing protein	Antitoxin
IUJ48_RS22390 (IUJ48_22340)	4898280..4898855	+	576	WP_058207229.1	PIN domain-containing protein	Toxin
IUJ48_RS22395 (IUJ48_22345)	4898872..4899786	+	915	WP_024067901.1	AAA family ATPase	-
IUJ48_RS22400 (IUJ48_22350)	4899783..4900253	+	471	WP_024067900.1	type III CBASS phage resistance system CD-NTase-associated protein Cap8	-
IUJ48_RS22405 (IUJ48_22355)	4900250..4900750	+	501	WP_024067899.1	type III CBASS phage resistance system CD-NTase-associated protein Cap7	-
IUJ48_RS22410 (IUJ48_22360)	4900750..4901652	+	903	WP_024067898.1	CBASS oligonucleotide cyclase	-
IUJ48_RS22415 (IUJ48_22365)	4901689..4902414	+	726	WP_024067897.1	CBASS effector endonuclease NucC	-
IUJ48_RS22420 (IUJ48_22370)	4902456..4903358	-	903	WP_024067896.1	bacteriophage abortive infection AbiH family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4838611..4955103	116492

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• SinI-like, DNA-binding domain (85-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21576.73 Da
• Isoelectric Point: 5.2880

>T181028 WP_058207229.1 NZ_CP064377:4898280-4898855 [Achromobacter xylosoxidans]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLIKRPDLNRAQVDRTSDLMDRAIPDGVVEGY
EELVAGLTLPDPDDRHVLAAAIRCGASVIVTFNERDFPNELLAAYGIEAQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDCFLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T181028 NZ_CP085799:c3014341-3013850 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGATCTCCACCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATAGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGCGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCTCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGGTTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTCGCTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGTGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTATCAGCGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17225.63 Da
• Isoelectric Point: 6.3803

>AT181028 WP_024067903.1 NZ_CP064377:4897813-4898283 [Achromobacter xylosoxidans]
MTTTAAQSKMTLPAAAEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHLVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT181028 NZ_CP085799:c3014631-3014338 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATTGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACTGACTTTGTGCTGGAAGCTGCCCGTGCTGCCGCCGAGGAAGCGCTGA
TCGAACAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGAGTTTCTCGTTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Structures

Toxin

• AlphaFold DB: A0A0D6GAF2