Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3379977..3380474 Replicon chromosome
Accession NZ_CP064376
Organism Shigella sonnei strain PartP-Ssonnei-RM8376

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag IUJ51_RS17255 Protein ID WP_001173343.1
Coordinates 3379977..3380150 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag IUJ51_RS17260 Protein ID WP_000398619.1
Coordinates 3380178..3380474 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IUJ51_RS17235 (3375129) 3375129..3376285 + 1157 WP_094096605.1 IS3-like element IS600 family transposase -
IUJ51_RS17240 (3376331) 3376331..3376588 + 258 Protein_3364 lysine decarboxylase LdcC -
IUJ51_RS17245 (3376647) 3376647..3378104 + 1458 WP_000856831.1 dipeptide/tripeptide permease DtpC -
IUJ51_RS17250 (3378341) 3378341..3379858 + 1518 WP_001295086.1 lysine--tRNA ligase -
IUJ51_RS17255 (3379977) 3379977..3380150 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
IUJ51_RS17260 (3380178) 3380178..3380474 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
IUJ51_RS17265 (3380701) 3380701..3380973 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
IUJ51_RS17270 (3380985) 3380985..3381215 - 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
IUJ51_RS17275 (3381396) 3381396..3383027 + 1632 WP_001216495.1 sensor histidine kinase -
IUJ51_RS17280 (3383024) 3383024..3383743 + 720 WP_000611285.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T181022 WP_001173343.1 NZ_CP064376:c3380150-3379977 [Shigella sonnei]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T181022 NZ_CP085799:c1527919-1527816 [Salmonella enterica subsp. enterica serovar Enteritidis]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT181022 WP_000398619.1 NZ_CP064376:c3380474-3380178 [Shigella sonnei]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT181022 NZ_CP085799:1527814-1527959 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References