Detailed information of TA system TA181020

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 2678439..2679276
• Replicon: chromosome
• Accession: NZ_CP064376
• Organism: Shigella sonnei strain PartP-Ssonnei-RM8376

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: IUJ51_RS13830
• Protein ID: WP_000227784.1
• Coordinates: 2678734..2679276 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: IUJ51_RS13825
• Protein ID: WP_001297137.1
• Coordinates: 2678439..2678750 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUJ51_RS13800 (2673459)	2673459..2674406	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
IUJ51_RS13805 (2674428)	2674428..2676419	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
IUJ51_RS13810 (2676409)	2676409..2677023	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
IUJ51_RS13815 (2677023)	2677023..2677352	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
IUJ51_RS13820 (2677364)	2677364..2678254	+	891	WP_000971327.1	heme o synthase	-
IUJ51_RS13825 (2678439)	2678439..2678750	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
IUJ51_RS13830 (2678734)	2678734..2679276	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
IUJ51_RS13835 (2679332)	2679332..2679898	-	567	Protein_2699	SEL1-like repeat protein	-
IUJ51_RS13840 (2679932)	2679932..2680629	+	698	WP_094096600.1	IS1-like element IS1A family transposase	-
IUJ51_RS13845 (2680646)	2680646..2681044	-	399	Protein_2701	SEL1-like repeat protein	-
IUJ51_RS13855 (2682229)	2682229..2683593	+	1365	WP_001000971.1	MFS transporter	-
IUJ51_RS13860 (2683721)	2683721..2684212	-	492	WP_001138904.1	nucleotide binding protein YajQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T181020 WP_000227784.1 NZ_CP064376:2678734-2679276 [Shigella sonnei]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T181020 NZ_CP085798:4608874-4609149 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGAGCCTGACTGGATTCTTATTTATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT181020 WP_001297137.1 NZ_CP064376:2678439-2678750 [Shigella sonnei]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT181020 NZ_CP085798:4608611-4608871 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGCTGCAAATGCGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGGACCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGCATAC
CCAATGAGTTAACGTCACGAACGATAGAAAATAGCGAAGCTGGCGTTGATATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3