Detailed information of TA system TA180970

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/Peptidase_M78-HTH_19
• Location: 392762..393456
• Replicon: chromosome
• Accession: NZ_CP064374
• Organism: Enterococcus faecalis strain PartL-Efaecalis-RM8376

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: A0A828QV40
• Locus tag: IUJ47_RS02025
• Protein ID: WP_002406159.1
• Coordinates: 393112..393456 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: A0A828QTI6
• Locus tag: IUJ47_RS02020
• Protein ID: WP_002406160.1
• Coordinates: 392762..393094 (+)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUJ47_RS01985 (388532)	388532..389308	-	777	WP_010816191.1	DnaD domain protein	-
IUJ47_RS01990 (389324)	389324..390139	-	816	WP_010816192.1	PD-(D/E)XK nuclease-like domain-containing protein	-
IUJ47_RS01995 (390102)	390102..391067	-	966	WP_010717378.1	RecT family recombinase	-
IUJ47_RS02000 (391168)	391168..391392	-	225	WP_002367281.1	hypothetical protein	-
IUJ47_RS02005 (391501)	391501..391917	-	417	WP_002370016.1	hypothetical protein	-
IUJ47_RS02010 (392111)	392111..392254	-	144	WP_002393055.1	hypothetical protein	-
IUJ47_RS02015 (392265)	392265..392441	-	177	WP_002364354.1	helix-turn-helix transcriptional regulator	-
IUJ47_RS02020 (392762)	392762..393094	+	333	WP_002406160.1	helix-turn-helix transcriptional regulator	Antitoxin
IUJ47_RS02025 (393112)	393112..393456	+	345	WP_002406159.1	ImmA/IrrE family metallo-endopeptidase	Toxin
IUJ47_RS02030 (393544)	393544..393975	+	432	WP_010784248.1	hypothetical protein	-
IUJ47_RS02035 (394085)	394085..395452	+	1368	WP_002406157.1	recombinase family protein	-
IUJ47_RS02040 (395474)	395474..396025	-	552	WP_002414896.1	DNA repair protein RadC	-
IUJ47_RS02045 (396081)	396081..396740	-	660	WP_002398690.1	HAD family hydrolase	-
IUJ47_RS02050 (396724)	396724..398046	-	1323	WP_002410879.1	folylpolyglutamate synthase/dihydrofolate synthase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	352333..395452	43119

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (24-89)

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-59)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13556.36 Da
• Isoelectric Point: 4.9882

>T180970 WP_002406159.1 NZ_CP064374:393112-393456 [Enterococcus faecalis]
MKSIKELVEEYNVELVFTTLHKKACFESEHGVIFVNQNLSTEEQEEAIYHEFKHVKDHADLMALYNIPIFRSKMEAEAEH
YMFECLIEKNDGQFNYSNVITHYNLKMGQETYLK

Nucleotide

• Length: 345 bp

>T180970 NZ_CP085795:c2438956-2438738 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12575.25 Da
• Isoelectric Point: 5.2423

>AT180970 WP_002406160.1 NZ_CP064374:392762-393094 [Enterococcus faecalis]
MTAFDRIKKITDRRGISINELENRVGISQNVLYGWKKKTPGGENLTKVAKYLNISTDYILGIVDNPEPFVEKKTDDLDDV
LDNVMSFDGEPLDDHDREVIRAYLKGRFGK

Nucleotide

• Length: 333 bp

>AT180970 NZ_CP085795:c2439358-2438984 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A828QV40

Antitoxin

• AlphaFold DB: A0A828QTI6