Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3083908..3084533 Replicon chromosome
Accession NZ_CP064368
Organism Klebsiella pneumoniae strain PartO-Kpneumoniae-RM8376

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag IUJ50_RS14930 Protein ID WP_002882817.1
Coordinates 3083908..3084291 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag IUJ50_RS14935 Protein ID WP_004150355.1
Coordinates 3084291..3084533 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IUJ50_RS14915 (3081274) 3081274..3082176 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
IUJ50_RS14920 (3082173) 3082173..3082808 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
IUJ50_RS14925 (3082805) 3082805..3083734 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
IUJ50_RS14930 (3083908) 3083908..3084291 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
IUJ50_RS14935 (3084291) 3084291..3084533 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
IUJ50_RS14940 (3084738) 3084738..3085655 + 918 WP_004150353.1 alpha/beta hydrolase -
IUJ50_RS14945 (3085669) 3085669..3086610 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
IUJ50_RS14950 (3086655) 3086655..3087092 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
IUJ50_RS14955 (3087089) 3087089..3087949 - 861 WP_002882807.1 virulence factor BrkB family protein -
IUJ50_RS14960 (3087943) 3087943..3088542 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T180953 WP_002882817.1 NZ_CP064368:c3084291-3083908 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T180953 NZ_CP085794:c2248380-2248123 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAATGCCTCCGGCGTCAGCGTCCGCCACATCAACAGCAAAACCCACATGACCACCTATTACTCACAAATCCCCAGCCT
GCATCTTAAGGGCGACTGGCTGGAAGAAGCGGGATTTAAGACCGGGCGCGGCGTCACCGTGAAGATTTCACAGGGGTGTA
TTGTGCTGATGGCGGACAGTAACGAGGAGCAGAAGCTGCGCGAGCAGCTTTATCAGGCTAAACAGGTGGTTAAGGGAATT
AAGGACGTGCTGGTTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT180953 WP_004150355.1 NZ_CP064368:c3084533-3084291 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT180953 NZ_CP085794:2248205-2248537 [Salmonella enterica subsp. enterica serovar Enteritidis]
TCCGCCATCAGCACAATACACCCCTGTGAAATCTTCACGGTGACGCCGCGCCCGGTCTTAAATCCCGCTTCTTCCAGCCA
GTCGCCCTTAAGATGCAGGCTGGGGATTTGTGAGTAATAGGTGGTCATGTGGGTTTTGCTGTTGATGTGGCGGACGCTGA
CGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTATCCGCCGGATGCTGGCTGATTGTGGAGCTCAGGGTG
AGCGAGTATGGGCGCGACATCCTGGCACTGAGCCCCCCTCTCCCCGGCCAGCTCCGGGCGGTGATGAGCAACCGGCTGCC
GGGGCCGTATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References