Detailed information of TA system TA180907

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 4493880..4494427
• Replicon: chromosome
• Accession: NZ_CP064354
• Organism: Pseudomonas fragi strain NL20W

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S6AKK9
• Locus tag: IS178_RS20410
• Protein ID: WP_016502317.1
• Coordinates: 4494152..4494427 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S6AY38
• Locus tag: IS178_RS20405
• Protein ID: WP_016502318.1
• Coordinates: 4493880..4494152 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS178_RS20380	4489073..4489471	+	399	WP_169716054.1	hypothetical protein	-
IS178_RS20385	4489502..4490764	-	1263	WP_016779384.1	hypothetical protein	-
IS178_RS20390	4490948..4492279	+	1332	WP_139803977.1	hypothetical protein	-
IS178_RS20395	4492706..4493446	+	741	WP_016779382.1	DUF2807 domain-containing protein	-
IS178_RS20400	4493453..4493866	+	414	WP_016502319.1	hypothetical protein	-
IS178_RS20405	4493880..4494152	+	273	WP_016502318.1	ribbon-helix-helix domain-containing protein	Antitoxin
IS178_RS20410	4494152..4494427	+	276	WP_016502317.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IS178_RS20415	4494571..4494840	-	270	WP_011077892.1	hypothetical protein	-
IS178_RS20420	4495235..4496215	-	981	WP_034113392.1	IS5 family transposase	-
IS178_RS20425	4496682..4497212	+	531	WP_016779380.1	hypothetical protein	-
IS178_RS20430	4497452..4498231	+	780	WP_169716048.1	DUF3987 domain-containing protein	-
IS178_RS20435	4498496..4499071	-	576	WP_016779378.1	YkgB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4476815..4511987	35172
	-	flank	IS/Tn	-	-	4495235..4496215	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (10-47)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10702.22 Da
• Isoelectric Point: 7.2020

>T180907 WP_016502317.1 NZ_CP064354:4494152-4494427 [Pseudomonas fragi]
MELKWTSKALSDIARLYEFLASVNQPAAARTVQQLTAAPSTLLANPRIGERLEEFDPRDVRRIQIGHYEMRYEIVDSTIY
LLRLWHTREDR

Nucleotide

• Length: 276 bp

>T180907 NZ_CP085789:4100-4387 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10074.43 Da
• Isoelectric Point: 5.7066

>AT180907 WP_016502318.1 NZ_CP064354:4493880-4494152 [Pseudomonas fragi]
MATTAKTRSVTAHVPVQLAEKVDLMAERLERSKNWIVKQALAAWIDQEEERSRLTREALADVDAGRVIDHQAVQAWAESL
STDTPLPVPR

Nucleotide

• Length: 273 bp

>AT180907 NZ_CP085789:4374-4688 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACACAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGATTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: S6AKK9

Antitoxin

• AlphaFold DB: S6AY38