Detailed information of TA system TA180906

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3817893..3819131
• Replicon: chromosome
• Accession: NZ_CP064354
• Organism: Pseudomonas fragi strain NL20W

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IS178_RS17230
• Protein ID: WP_029611301.1
• Coordinates: 3818556..3819131 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: IS178_RS17225
• Protein ID: WP_081481025.1
• Coordinates: 3817893..3818450 (+)
• Length: 186 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS178_RS17200	3812960..3813256	+	297	WP_016779995.1	hypothetical protein	-
IS178_RS17205	3813321..3813839	-	519	WP_016779994.1	DUF4142 domain-containing protein	-
IS178_RS17210	3814009..3814350	+	342	WP_016779993.1	hypothetical protein	-
IS178_RS17215	3814678..3815733	+	1056	WP_016779992.1	fatty acid desaturase	-
IS178_RS17220	3815916..3817061	+	1146	WP_016779991.1	EstA family serine hydrolase	-
IS178_RS17225	3817893..3818450	+	558	WP_081481025.1	helix-turn-helix domain-containing protein	Antitoxin
IS178_RS17230	3818556..3819131	+	576	WP_029611301.1	HD domain-containing protein	Toxin
IS178_RS17235	3819210..3819449	-	240	Protein_3377	Presumed portal vertex protein	-
IS178_RS17240	3819449..3820091	-	643	Protein_3378	terminase	-
IS178_RS17245	3820113..3820262	+	150	WP_016779986.1	hypothetical protein	-
IS178_RS17250	3820364..3821575	-	1212	WP_016779985.1	molybdopterin-synthase adenylyltransferase MoeB	-
IS178_RS17255	3821640..3822473	-	834	WP_037000995.1	hypothetical protein	-
IS178_RS17260	3822478..3822753	-	276	WP_016779983.1	MoaD/ThiS family protein	-
IS178_RS17265	3822756..3823931	-	1176	WP_016779982.1	acyl-CoA dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3814678..3823931	9253

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (9-184)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-64)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21590.62 Da
• Isoelectric Point: 4.6835

>T180906 WP_029611301.1 NZ_CP064354:3818556-3819131 [Pseudomonas fragi]
MDIEKIKGRLEFLREAEKLKDVLRSAHTSSGRTESTAEHSWRLCLMAVVFGDYLTGLDRLKMLKMCVIHDLGEAISGDIP
AINKDSFPNKSEQERSDLILLTNTLDESLKAEILALWDDYENALSPEAVAVKALDKLETMLQHNQGKNPADFDYEFNLAY
GKKYTDAAPLFEALRNIIDEETKANMLLNPK

Nucleotide

• Length: 576 bp

>T180906 NZ_CP085788:c3581181-3580792 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGCTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATCAGTCACAGAACACCGTT
TCTCGCGTCAAAATTTTAAAAATAATTGATTATCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTCACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin

Protein

• Length: 186 a.a.
• Molecular weight: 20510.66 Da
• Isoelectric Point: 7.4056

>AT180906 WP_081481025.1 NZ_CP064354:3817893-3818450 [Pseudomonas fragi]
MPDMLLKPSLPGVMLRRWRLLHQVKQSHAAELFSVTQSTISRWESGVLDLDSLAHEKLEKLLAARLTTAADQALGRMVRE
SSRPVHLICDLTHRLLACSSSRAAQFASPVSELLGSSLWRFATPTIISMEADLDRLGWREAFAPPALEFLTGDNHSSIVP
ISQSLCRWTQIPLSDGATTRLVESL

Nucleotide

• Length: 558 bp

>AT180906 NZ_CP085788:c3581445-3581188 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ATCCTGATTTACTCGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA