Detailed information of TA system TA180905

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 1732286..1734003
• Replicon: chromosome
• Accession: NZ_CP064354
• Organism: Pseudomonas fragi strain NL20W

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: IS178_RS07880
• Protein ID: WP_016782260.1
• Coordinates: 1733119..1734003 (-)
• Length: 295 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: IS178_RS07875
• Protein ID: WP_016782259.1
• Coordinates: 1732286..1733122 (-)
• Length: 279 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS178_RS07855	1727389..1728885	-	1497	WP_016782255.1	aldehyde dehydrogenase	-
IS178_RS07860	1728998..1729801	-	804	WP_016782256.1	aspartate dehydrogenase	-
IS178_RS07865	1729812..1731494	-	1683	WP_016782257.1	thiamine pyrophosphate-binding protein	-
IS178_RS07870	1731487..1732284	-	798	WP_016782258.1	SDR family oxidoreductase	-
IS178_RS07875	1732286..1733122	-	837	WP_016782259.1	IclR family transcriptional regulator	Antitoxin
IS178_RS07880	1733119..1734003	-	885	WP_016782260.1	alpha/beta hydrolase	Toxin
IS178_RS07885	1734008..1734550	-	543	WP_016782261.1	cupin domain-containing protein	-
IS178_RS07890	1734564..1735328	-	765	WP_139803916.1	SDR family oxidoreductase	-
IS178_RS07895	1735328..1736104	-	777	WP_016782262.1	ABC transporter permease	-
IS178_RS07900	1736133..1737134	-	1002	WP_016782263.1	ABC transporter substrate-binding protein	-
IS178_RS07905	1737172..1738023	-	852	WP_016782264.1	ABC transporter ATP-binding protein	-
IS178_RS07910	1738651..1738794	+	144	WP_016782265.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (47-278)

Antitoxin

• Transcription regulator IclR, N-terminal (19-68)

Sequences

Toxin

Protein

• Length: 295 a.a.
• Molecular weight: 30881.29 Da
• Isoelectric Point: 7.6853

>T180905 WP_016782260.1 NZ_CP064354:c1734003-1733119 [Pseudomonas fragi]
MLQTTDHLDPSLTADLEAQLGLNFPLRRLDLAGGQQALRECGRGPAIVLLHGIGSGAASWLAVAQLLGQRARVIAWDAPG
YGQSSPLRSLAPDASDYAGRLGQMLDALGVERCVLVGHSLGAITATAFASGAQQQRVSRLVLISPAQGYGHPSRQAQGRA
VRKKRLADLEHPGIQAMARQRSACMLSPGASPGDHAWVRWNMAQLNPEGYRQAIELLCGEDLLRQPPLSMPCDVHCGAAD
GITTPDSCQLLARALGASFSLMTGAGHASPIEQPYAVAGRLALAIEHSLTGASL

Nucleotide

• Length: 885 bp

>T180905 NZ_CP085788:c530289-529894 [Acinetobacter baumannii]
ATGATTTACCTCTTAGACACAAATATTTGTATCTACGTGATCAATCATAAACCACAGCAAGTTTTTGAACGCTTTAAGCA
ATATCAGCTTGGACAGTTAGCTATTTCTAGCATAACCGCGTCAGAGCTGGCTTTTGGCGTTGAAAAGTCTGGATCTGAAC
GCAATAAGCAAGCTCTGAACAAGTTTTTATCACCCTTAGATATTTTACCCTACGATGAGCAAGCGATTTGGCATTACGCT
AAATTACGTCAAGATCTCCAATCAACAGGTAAAACTATTGGTAGTTTAGATATGCTAATAGCAGCTCATGCCCTCGCTTT
AGATGTTGTACTAGTCACAAACAACATCAAGGAATTTGAGCGTATAGATGGGTTAAAGTTAGAGAACTGGACCTAA

Antitoxin

Protein

• Length: 279 a.a.
• Molecular weight: 30785.20 Da
• Isoelectric Point: 6.3501

>AT180905 WP_016782259.1 NZ_CP064354:c1733122-1732286 [Pseudomonas fragi]
MTDSELPKDAQDKYIVPGLERGLLLLCEFTRSNRTLTAPELARRLALPRSTIFRLLTTLETMGFVTRSGNEYRLGMSVLR
LGFEYLASLELTELGQPLLARLCDRVNYPSNLVVRDGRSIVYVAKVSPPSVFSSAVNVGTRLPAHATVLGRILLEDLSLS
ELRELYPEEHLEQHSPCTPKTALELFDMVQADRQRGFVSGEGFFESSISTVAAPVRDESSRIVAALGVTVPTAQIAHVNF
NELLLQVRRSADELSRLLNYNSGAANDGHNRVTALMRD

Nucleotide

• Length: 837 bp

>AT180905 NZ_CP085788:c530516-530286 [Acinetobacter baumannii]
ATGGAAACCGCTAAAGTTTTTCAAACAGGTCGTAGTCAAGCTGTCCGTTTACCTAAAGCATTTCGCTTTAATGGTACTGA
AGTTGCTATCAAGAGTTTTGGCCGTGGTGTCTTATTAATGCCCATCGAAAACCCATGGGACATCATGTTAGAAGCAATTA
ACGAATTTGAAGTAGGATTTCAGCTTGAACGAGCTGATCAGGGCGAGCAATTACGTGAGGACTTCAAATGA